Rabbit ARL2 Polyclonal Antibody | anti-ARL2 antibody

ARL2 Antibody

Cat. Num. AAA9630304

• Gene Names: ARL2; ARFL2
• Reactivity: Human, Mouse, Rat
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: ARL2; Polyclonal Antibody; ARL2 Antibody; ADP ribosylation factor like 2; ADP-ribosylation factor-like protein 2; ARFL2; Arl184; Arl2; ARL2_HUMAN; anti-ARL2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: ARL2 Antibody detects endogenous levels of ARL2.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-ARL2 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human ARL2.
• Conjugation: Unconjugated
• Similarity: Belongs to the small GTPase superfamily. Arf family.
• Post Translational Modifications: Not N-myristoylated.
• Subunit Structure: Found in a complex with ARL2, ARL2BP and SLC25A6. Found in a complex with at least ARL2, PPP2CB, PPP2R1A, PPP2R2A, PPP2R5E and TBCD. Found in a complex with ARL2, ARL2BP and SLC25A4. The GTP-bound form interacts with PDE6D. Interacts with ELMOD2. The GTP-bound form interacts with ARL2BP. Interacts, preferentially in its GDP-bound state, with TBCD. Interacts with UNC119.
• Function: Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). GTP-binding protein that does not act as an allosteric activator of the cholera toxin catalytic subunit. Regulates formation of new microtubules and centrosome integrity. Prevents the TBCD-induced microtubule destruction. Participates in association with TBCD, in the disassembly of the apical junction complexes. Antagonizes the effect of TBCD on epithelial cell detachment and tight and adherens junctions disassembly. Together with ARL2, plays a role in the nuclear translocation, retention and transcriptional activity of STAT3. Component of a regulated secretory pathway involved in Ca(2+)-dependent release of acetylcholine. Required for normal progress through the cell cycle.
• Note: The complex formed with ARL2BP, ARL2 and SLC25A6 is expressed in mitochondria. The complex formed with ARL2BP, ARL2 and SLC25A4 is expressed in mitochondria (By similarity). Not detected in the Golgi, nucleus and on the mitotic spindle. Centrosome-associated throughout the cell cycle. Not detected to interphase microtubules.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-ARL2 antibody

Mitochondrion intermembrane space; Cytoplasm; Cytoskeleton; Microtubule organizing center; Centrosome; Nucleus; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 116241255
• NCBI GeneID: 402
• UniProt Accession #: P36404; Q9BUK8; G3V184
• Molecular Weight: 184
• NCBI Official Full Name: ADP-ribosylation factor-like protein 2
• NCBI Official Synonym Full Names: ADP-ribosylation factor-like 2
• NCBI Official Symbol: ARL2
• NCBI Official Synonym Symbols: ARFL2
• NCBI Protein Information: ADP-ribosylation factor-like protein 2
• UniProt Protein Name: ADP-ribosylation factor-like protein 2
• Protein Family: ADP-ribosylation factor-like protein
• UniProt Gene Name: ARL2
• UniProt Entry Name: ARL2_HUMAN

NCBI Description

This gene encodes a small GTP-binding protein of the RAS superfamily which functions as an ADP-ribosylation factor (ARF). The encoded protein is one of a functionally distinct group of ARF-like genes. [provided by RefSeq, Jul 2008]

Uniprot Description

ARL2: Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). GTP-binding protein that does not act as an allosteric activator of the cholera toxin catalytic subunit. Regulates formation of new microtubules and centrosome integrity. Prevents the TBCD-induced microtubule destruction. Participates in association with TBCD, in the disassembly of the apical junction complexes. Antagonizes the effect of TBCD on epithelial cell detachment and tight and adherens junctions disassembly. Together with ARL2, plays a role in the nuclear translocation, retention and transcriptional activity of STAT3. Component of a regulated secretory pathway involved in Ca(2+)-dependent release of acetylcholine. Required for normal progress through the cell cycle. Belongs to the small GTPase superfamily. Arf family.

Protein type: G protein, monomeric, ARF; Mitochondrial; G protein, monomeric

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: centrosome; mitochondrial intermembrane space; nucleus; lateral plasma membrane

Molecular Function: GTPase activity; protein binding; GDP binding; GTP binding; GTPase inhibitor activity

Biological Process: tubulin folding; centrosome organization and biogenesis; metabolic process; small GTPase mediated signal transduction; positive regulation of microtubule polymerization; maintenance of protein localization in nucleus; cell cycle; regulation of microtubule polymerization