Rabbit ARIH2 Polyclonal Antibody | anti-ARIH2 antibody

ARIH2 Antibody

Cat. Num. AAA9611583

• Gene Names: ARIH2; ARI2; TRIAD1
• Reactivity: Human, Mouse, Rat; Predicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
• Applications: Western Blot
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: ARIH2; Polyclonal Antibody; ARIH2 Antibody; HT005; all trans retinoic acid inducible RING finger; Ari 2; Ari-2; ARI2; ARI2_HUMAN; Ariadne 2; Ariadne 2 protein homolog; ariadne homolog 2 (Drosophila); ariadne homolog 2; Ariadne RBR E3 ubiquitin protein ligase 2; ARIH 2; Arih2; E3 ubiquitin protein ligase ARIH2; E3 ubiquitin-protein ligase ARIH2; protein ariadne 2 homolog; Protein ariadne-2 homolog; Triad 1; TRIAD1; Triad1 protein; UIP48; anti-ARIH2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ARIH2 Antibody detects endogenous levels of ARIH2.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 493

• Applicable Applications for anti-ARIH2 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A synthesized peptide derived from human ARIH2.
• Tissue Specificity: Widely expressed with higher expression in granulocytes.
• Fragment: Fab Fragment
• Conjugate: Unconjugated
• Subcellular Location: Nucleus
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Immunofluorescence (IF)/Immunocytochemistry (ICC)

(staining HeLa cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody , diluted at 1/600, was used as secondary antibod)

Western Blot (WB)

(Western blot analysis of extracts from HeLa cells, using ARIH2 Antibody. The lane on the left was treated with blocking peptide.)

Western Blot (WB)

(Western blot analysis of extracts from MCF7, using ARIH2 Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-ARIH2 antibody

Description: E3 ubiquitin-protein ligase, which catalyzes ubiquitination of target proteins together with ubiquitin-conjugating enzyme E2 UBE2L3 (PubMed:16118314, PubMed:17646546, PubMed:19340006, PubMed:24076655). Acts as an atypical E3 ubiquitin-protein ligase by working together with cullin-5-RING ubiquitin ligase complex (ECS complex, also named CRL5 complex) and initiating ubiquitination of ECS substrates: associates with ECS complex and specifically mediates addition of the first ubiquitin on ECS targets (By similarity). The initial ubiquitin is then elongated (By similarity). E3 ubiquitin-protein ligase activity is activated upon binding to neddylated form of the ECS complex (PubMed:24076655). Mediates 'Lys-6', 'Lys-48'-and 'Lys-63'-linked polyubiquitination (PubMed:16118314, PubMed:17646546, PubMed:19340006). May play a role in myelopoiesis (PubMed:19340006).

Post Translational Modifications: Ubiquitinated. Ubiquitination promotes proteasomal degradation.

Subunit Structure: Interacts (via RING-type zinc finger 1) with UBE2L3 (PubMed:16118314, PubMed:19340006, PubMed:24076655). Interacts (via RING-type zinc finger 2) with UBE2N (PubMed:19340006). Interacts with neddylated CUL5 (PubMed:24076655). Interacts (via RING-type 2) with GFI1B (PubMed:17646546). Interacts with GFI1; prevents its ubiquitination and proteasomal degradation (PubMed:17646546).

Similarity: Members of the RBR family are atypical E3 ligases. They interact with the E2 conjugating enzyme UBE2L3 and function like HECT-type E3 enzymes: they bind E2s via the first RING-type zinc finger, but require an obligate trans-thiolation step during the ubiquitin transfer, requiring a conserved active site Cys residue in the second RING-type zinc finger. The active site probably forms a thioester intermediate with ubiquitin taken from the active-site cysteine of the E2 before ultimately transferring it to a Lys residue on the substrate. The Ariadne domain inhibits activity by masking the second RING-type zinc finger that contains the active site. Belongs to the RBR family. Ariadne subfamily.

NCBI and Uniprot Product Information

• NCBI GI #: 5453557
• NCBI GeneID: 10425
• NCBI Accession #: NP_006312
• NCBI GenBank Nucleotide #: NP_006312.1
• UniProt Accession #: O95376
• Molecular Weight: Observed: 59 kDa; Predicted: 58 kDa
• NCBI Official Full Name: E3 ubiquitin-protein ligase ARIH2 isoform a
• NCBI Official Synonym Full Names: ariadne RBR E3 ubiquitin protein ligase 2
• NCBI Official Symbol: ARIH2
• NCBI Official Synonym Symbols: ARI2; TRIAD1
• NCBI Protein Information: E3 ubiquitin-protein ligase ARIH2
• UniProt Protein Name: E3 ubiquitin-protein ligase ARIH2
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: ARIH2
• UniProt Synonym Gene Names: ARI2; TRIAD1; ARI-2; Protein ariadne-2 homolog
• UniProt Entry Name: ARI2_HUMAN

NCBI Description

The protein encoded by this gene is an E3 ubiquitin-protein ligase that polyubiquitinates some proteins, tagging them for degradation. The encoded protein upregulates p53 in some cancer cells and may inhibit myelopoiesis. Several transcript variants encoding different isoforms have been found for this gene, although the full-length nature of some of them have not been determined yet. [provided by RefSeq, Nov 2015]