Western Blot (WB)
(Figure 1. Western blot analysis of ARID5A using anti-ARID5A antibody (MBS1753839).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human placenta tissue lysatesLane 2: human K562 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-ARID5A antigen affinity purified polyclonal antibody (Catalog # MBS1753839) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for ARID5A at approximately 64KD. The expected band size for ARID5A is at 64KD. )
Rabbit anti-Human ARID5A Polyclonal Antibody | anti-ARID5A antibody
Anti-ARID5A Antibody
Gene Names
ARID5A; MRF1; MRF-1; RP11-363D14
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Immunogen affinity purified.
Synonyms
ARID5A; Polyclonal Antibody; Anti-ARID5A Antibody; MRF1; AT-rich interactive domain-containing protein 5A; ARID domain-containing protein 5A; Modulator recognition factor 1; MRF-1; AT-rich interaction domain 5A; anti-ARID5A antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Rabbit IgG polyclonal antibody for ARID5A detection.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized. Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Applicable Applications for anti-ARID5A antibody
Western Blot (WB), Direct ELISA (EIA)
Application Notes
WB: 0.25-0.5ug/ml|Human|
Direct ELISA: 0.1-0.5ug/ml|Human|
Direct ELISA: 0.1-0.5ug/ml|Human|
Immunogen
E Coli-derived human ARID5A recombinant protein (Position: R55-L594).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Recommended Detection Systems
Recommended Detection Systems
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Western Blot (WB)
(Figure 1. Western blot analysis of ARID5A using anti-ARID5A antibody (MBS1753839).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human placenta tissue lysatesLane 2: human K562 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-ARID5A antigen affinity purified polyclonal antibody (Catalog # MBS1753839) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for ARID5A at approximately 64KD. The expected band size for ARID5A is at 64KD. )
Western Blot (WB)
(Figure 1. Western blot analysis of ARID5A using anti-ARID5A antibody (MBS1753839).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human placenta tissue lysatesLane 2: human K562 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-ARID5A antigen affinity purified polyclonal antibody (Catalog # MBS1753839) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for ARID5A at approximately 64KD. The expected band size for ARID5A is at 64KD. )
Related Product Information for anti-ARID5A antibody
Members of the ARID protein family, including ARID5A, have diverse functions but all appear to play important roles in development, tissue-specific gene expression, and regulation of cell growth.
References
1. Amano, K., Hata, K., Muramatsu, S., Wakabayashi, M., Takigawa, Y., Ono, K., Nakanishi, M., Takashima, R., Kogo, M., Matsuda, A., Nishimura, R., Yoneda, T. Arid5a cooperates with Sox9 to stimulate chondrocyte-specific transcription. Molec. Biol. Cell 22: 1300-1311, 2011.
2. Georgescu, S. P., Li, J. H., Lu, Q., Karas, R. H., Brown, M., Mendelsohn, M. E. Modulator recognition factor 1, an AT-rich interaction domain family member, is a novel corepressor for estrogen receptor alpha. Molec. Endocr. 19: 2491-2501, 2005.
3. Gross, M. B. Personal Communication. Baltimore, Md. 2/26/2015.
2. Georgescu, S. P., Li, J. H., Lu, Q., Karas, R. H., Brown, M., Mendelsohn, M. E. Modulator recognition factor 1, an AT-rich interaction domain family member, is a novel corepressor for estrogen receptor alpha. Molec. Endocr. 19: 2491-2501, 2005.
3. Gross, M. B. Personal Communication. Baltimore, Md. 2/26/2015.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
64,074 Da
NCBI Official Full Name
AT-rich interactive domain-containing protein 5A
NCBI Official Synonym Full Names
AT rich interactive domain 5A (MRF1-like)
NCBI Official Symbol
ARID5A
NCBI Official Synonym Symbols
MRF1; MRF-1; RP11-363D14
NCBI Protein Information
AT-rich interactive domain-containing protein 5A; ARID domain-containing protein 5A; RFVG5814; modulator recognition factor 1; modulator recognition factor I
UniProt Protein Name
AT-rich interactive domain-containing protein 5A
Protein Family
UniProt Gene Name
ARID5A
UniProt Synonym Gene Names
MRF1; ARID domain-containing protein 5A; MRF-1
UniProt Entry Name
ARI5A_HUMAN
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Product Notes
The ARID5A arid5a (Catalog #AAA1753839) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-ARID5A Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ARID5A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Direct ELISA (EIA). WB: 0.25-0.5ug/ml|Human| Direct ELISA: 0.1-0.5ug/ml|Human|. Researchers should empirically determine the suitability of the ARID5A arid5a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ARID5A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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