Human Glutaredoxin 1 (GLRX) ELISA Kit | GLRX elisa kit
Human Glutaredoxin 1 (GLRX) ELISA Kit
Reactivity
Human
Synonyms
Glutaredoxin 1 (GLRX); Human Glutaredoxin 1 (GLRX) ELISA Kit; GLRX elisa kit
Reactivity
Human
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
250-5000pg/mL
Sensitivity
1.0pg/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for GLRX elisa kit
Intended Uses: This GLRX ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human GLRX.
Principle of the Assay: GLRX ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-GLRX antibody and an GLRX-HRP conjugate. The assay sample and buffer are incubated together with GLRX-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GLRX concentration since GLRX from samples and GLRX-HRP conjugate compete for the anti-GLRX antibody binding site. Since the number of sites is limited, as more sites are occupied by GLRX from the sample, fewer sites are left to bind GLRX-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GLRX concentration in each sample is interpolated from this standard curve.
Principle of the Assay: GLRX ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-GLRX antibody and an GLRX-HRP conjugate. The assay sample and buffer are incubated together with GLRX-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GLRX concentration since GLRX from samples and GLRX-HRP conjugate compete for the anti-GLRX antibody binding site. Since the number of sites is limited, as more sites are occupied by GLRX from the sample, fewer sites are left to bind GLRX-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GLRX concentration in each sample is interpolated from this standard curve.
