Rabbit anti-Human ARHGEF3 Polyclonal Antibody | anti-ARHGEF3 antibody

ARHGEF3, ID (ARHGEF3, Rho guanine nucleotide exchange factor 3, Exchange factor found in platelets and leukemic and neuronal tissues)

Cat. Num. AAA6008490

• Gene Names: ARHGEF3; GEF3; STA3; XPLN
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: ARHGEF3; Polyclonal Antibody; ID (ARHGEF3; Rho guanine nucleotide exchange factor 3; Exchange factor found in platelets and leukemic and neuronal tissues); Anti -ARHGEF3; anti-ARHGEF3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-ARHGEF3 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:50-100
• Immunogen: ARHGEF3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 218-249 amino acids from the Central region of human ARHGEF3.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(ARHGEF3 Antibody (Center) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the ARHGEF3 antibody detected the ARHGEF3 protein (arrow).)

Immunohistochemistry (IHC)

(ARHGEF3 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ARHGEF3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-ARHGEF3 antibody

Rho-like GTPases are involved in a variety of cellular processes, and they are activated by binding GTP and inactivated by conversion of GTP to GDP by their intrinsic GTPase activity. Guanine nucleotide exchange factors (GEFs) accelerate the GTPase activity of Rho GTPases by catalyzing their release of bound GDP. This gene encodes a guanine nucleotide exchange factor, which specifically activates two members of the Rho GTPase family: RHOA and RHOB, both of which have a role in bone cell biology. It has been identified that genetic variation in this gene plays a role in the determination of bone mineral density (BMD), indicating the implication of this gene in postmenopausal osteoporosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-ARHGEF3 antibody

Antibodies; Abs to GTPase; Rab; Ras; Rho Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 71043417
• NCBI GeneID: 50650
• UniProt Accession #: Q9NR81; Q4FZB6; Q4QQI5; Q4QQQ0; Q59F00; Q6NUN3; Q7Z4U2; Q7Z5T2; Q9H7T4; A8K5U7
• Molecular Weight: 59,783 Da
• NCBI Official Full Name: ARHGEF3 protein
• NCBI Official Synonym Full Names: Rho guanine nucleotide exchange factor (GEF) 3
• NCBI Official Symbol: ARHGEF3
• NCBI Official Synonym Symbols: GEF3; STA3; XPLN
• NCBI Protein Information: rho guanine nucleotide exchange factor 3; RhoGEF protein; 59.8 kDA protein; exchange factor found in platelets and leukemic and neuronal tissues, XPLN
• UniProt Protein Name: Rho guanine nucleotide exchange factor 3
• Protein Family: Rho guanine nucleotide exchange factor
• UniProt Gene Name: ARHGEF3
• UniProt Synonym Gene Names: XPLN
• UniProt Entry Name: ARHG3_HUMAN

NCBI Description

Rho-like GTPases are involved in a variety of cellular processes, and they are activated by binding GTP and inactivated by conversion of GTP to GDP by their intrinsic GTPase activity. Guanine nucleotide exchange factors (GEFs) accelerate the GTPase activity of Rho GTPases by catalyzing their release of bound GDP. This gene encodes a guanine nucleotide exchange factor, which specifically activates two members of the Rho GTPase family: RHOA and RHOB, both of which have a role in bone cell biology. It has been identified that genetic variation in this gene plays a role in the determination of bone mineral density (BMD), indicating the implication of this gene in postmenopausal osteoporosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ARHGEF3: Acts as guanine nucleotide exchange factor (GEF) for RhoA and RhoB GTPases. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: GAPs; Motility/polarity/chemotaxis; GAPs, Rac/Rho

Chromosomal Location of Human Ortholog: 3p14.3

Cellular Component: intracellular; cytosol

Molecular Function: Rho guanyl-nucleotide exchange factor activity

Biological Process: regulation of small GTPase mediated signal transduction; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; small GTPase mediated signal transduction; Rho protein signal transduction