Western Blot (WB)
(Western blot analysis of ARHGAP20 in rat brain lysate. Lane 1 shows pre-immune sera. Lane 2 shows tested at 8ug/ml.)
Rabbit anti-Human, Mouse ARHGAP20 Polyclonal Antibody | anti-ARHGAP20 antibody
ARHGAP20 (Rho GTPase-activating Protein 20, Rho-type GTPase-activating Protein 20, KIAA1391)
Gene Names
ARHGAP20; RARHOGAP
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
ARHGAP20; Polyclonal Antibody; ARHGAP20 (Rho GTPase-activating Protein 20; Rho-type GTPase-activating Protein 20; KIAA1391); Anti -ARHGAP20 (Rho GTPase-activating Protein 20; anti-ARHGAP20 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human ARHGAP20. Species Crossreactivity
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Applicable Applications for anti-ARHGAP20 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in Western Blot and Immunohistochemistry.
Dilution: Western Blot: 5-8ug/ml
Immunohistochemistry (paraffin): 4-8ug/ml
Dilution: Western Blot: 5-8ug/ml
Immunohistochemistry (paraffin): 4-8ug/ml
Immunogen
Synthetic peptide corresponding to aa570-620 of human ARHGAP20.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of ARHGAP20 in rat brain lysate. Lane 1 shows pre-immune sera. Lane 2 shows tested at 8ug/ml.)
Western Blot (WB)
(Western blot analysis of ARHGAP20 in rat brain lysate. Lane 1 shows pre-immune sera. Lane 2 shows tested at 8ug/ml.)
Immunohistochemistry (IHC)
(IHC staining of mouse brain using Rho GTPase activating protein at 5ug/ml.)
Immunohistochemistry (IHC)
(IHC staining of mouse brain using Rho GTPase activating protein at 5ug/ml.)
Immunohistochemistry (IHC)
(IHC staining of mouse brain using control (rabbit Ig) at 5ug/ml.)
Immunohistochemistry (IHC)
(IHC staining of mouse brain using control (rabbit Ig) at 5ug/ml.)
Related Product Information for anti-ARHGAP20 antibody
ARHGAP20 is a potent GTPase-activating protein (GAP) and convert Rho-type GTPases into an inactive GDP-bound state. It contains a pleckstrin homology (PH) domain, a Ras-associating domain, a Rho-GAP domain and two Annexin-like (ANXL) repeats. ARHGAP20 is a direct downstream target of Rap1 in the neurite outgrowth while it's over expression leads to inactivation of Rho for promoting the neurite outgrowth in a Rap1-dependent manner. ARHGAP20 is a tumor suppressor gene and is inactivated by deletion in breast cancer and also by chromosomal translocation in B-cell chronic lymphocytic leukemia. Reports suggest that it may take part in rearrangements of the cytoskeleton and cell signaling events that occur during spermatogenesis. It is expressed predominantly in human brain, liver, ovary and spinal cord while low expression is found in lymph nodes and fetal liver.
Product Categories/Family for anti-ARHGAP20 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
132,608 Da
NCBI Official Full Name
ARHGAP20 protein
NCBI Official Synonym Full Names
Rho GTPase activating protein 20
NCBI Official Symbol
ARHGAP20
NCBI Official Synonym Symbols
RARHOGAP
NCBI Protein Information
rho GTPase-activating protein 20; rho-type GTPase-activating protein 20; RA and RhoGAP domain containing protein; rho GTPase activating protein 20 variant 2
UniProt Protein Name
Rho GTPase-activating protein 20
Protein Family
UniProt Gene Name
ARHGAP20
UniProt Synonym Gene Names
KIAA1391
UniProt Entry Name
RHG20_HUMAN
Uniprot Description
ARHGAP20: GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state. A chromosomal aberration involving ARHGAP20 may be a cause of B-cell chronic lymphocytic leukemia (B-CLL). Translocation t(X;11)(q21;q23) with BRWD3 does not result in fusion transcripts but disrupts both genes. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Tumor suppressor; GAPs, Rac/Rho; GAPs
Chromosomal Location of Human Ortholog: 11q23.1
Cellular Component: cytosol
Molecular Function: GTPase activator activity
Biological Process: regulation of small GTPase mediated signal transduction; small GTPase mediated signal transduction; positive regulation of GTPase activity
Research Articles on ARHGAP20
Similar Products
Product Notes
The ARHGAP20 arhgap20 (Catalog #AAA6003846) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ARHGAP20 (Rho GTPase-activating Protein 20, Rho-type GTPase-activating Protein 20, KIAA1391) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ARHGAP20 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in Western Blot and Immunohistochemistry. Dilution: Western Blot: 5-8ug/ml Immunohistochemistry (paraffin): 4-8ug/ml. Researchers should empirically determine the suitability of the ARHGAP20 arhgap20 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ARHGAP20, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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