Rabbit anti-Human Argonaute 4/Ago4 Polyclonal Antibody | anti-Ago4 antibody

Anti-Argonaute 4/Ago4 Antibody, Rabbit Polyclonal

Cat. Num. AAA8100785

• Gene Names: AGO4; EIF2C4
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: Argonaute 4/Ago4; Polyclonal Antibody; Anti-Argonaute 4/Ago4 Antibody; Rabbit Polyclonal; Ago4 Antibody; Rabbit PAb; Antigen Affinity Purified; argonaute 4; RISC catalytic component; Anti-EIF2C4 Antibody; anti-Ago4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human Ago4
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-Ago4 antibody: Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: IHC-P: 1:100-1:500
• Immunogen: A synthetic peptide corresponding to the C-terminus of the Human Ago4
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human Ago4, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human AGO4 in human testis with rabbit polyclonal antibody (1:300, formalin-fixed paraffin embedded sections).)

Related Product Information for anti-Ago4 antibody

The four mammalian Argonaute family members are thought to share redundant functions in the microRNA pathway, yet only AGO2 possesses the catalytic "slicer" function required for RNAi. Argonaute (AGO) proteins are core components of small RNA-induced silencing pathways, which negatively regulate gene expression in a sequence-specific manner. In the post-transcriptional gene silencing (PTGS) pathway, AGO proteins bind to small RNAs such as miRNAs, tasiRNAs, or piRNAs and mediate gene silencing through degradation and/or translational suppression of the target mRNAs. AGO proteins are highly conserved and present in various life forms ranging from Archaea to humans. Each AGO protein is characterized by three conserved domains: PAZ, MID, and PIWI. The PAZ domain recognizes and binds the 3-end of small RNAs.

References

Duan CG, et al. (2015) Specific but interdependent functions for arabidopsis ago4 and ago6 in rna-directed DNA methylation. Embo j 34 (5): 581-592.

NCBI and Uniprot Product Information

• NCBI GI #: 29029593
• NCBI GeneID: 192670
• NCBI Accession #: NP_060099.2
• NCBI GenBank Nucleotide #: NM_017629.3
• UniProt Accession #: Q9HCK5; A7MD27
• Molecular Weight: 97,097 Da
• NCBI Official Full Name: protein argonaute-4
• NCBI Official Synonym Full Names: argonaute 4, RISC catalytic component
• NCBI Official Symbol: AGO4
• NCBI Official Synonym Symbols: EIF2C4
• NCBI Protein Information: protein argonaute-4
• UniProt Protein Name: Protein argonaute-4
• Protein Family: Protein argonaute
• UniProt Gene Name: AGO4
• UniProt Synonym Gene Names: EIF2C4; KIAA1567; Argonaute4; hAgo4; eIF-2C 4; eIF2C 4
• UniProt Entry Name: AGO4_HUMAN

NCBI Description

This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic containing PAZ and PIWI domains, and it may play a role in short-interfering-RNA-mediated gene silencing. This gene is located on chromosome 1 in a cluster of closely related family members including argonaute 3, and eukaryotic translation initiation factor 2C, 1. [provided by RefSeq, Jul 2008]

Uniprot Description

AGO4: Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for RNA-directed transcription and replication of the human hapatitis delta virus (HDV). Interacts with EIF4B, IMP8, PRMT5, TNRC6A and TNRC6B. Belongs to the argonaute family. Ago subfamily.

Protein type: Translation; Translation initiation

Chromosomal Location of Human Ortholog: 1p34

Cellular Component: cytoplasm; cytosol; membrane

Molecular Function: double-stranded RNA binding; miRNA binding; protein binding; single-stranded RNA binding

Biological Process: miRNA-mediated gene silencing, miRNA loading onto RISC; miRNA-mediated gene silencing, mRNA cleavage; miRNA-mediated gene silencing, negative regulation of translation; miRNA-mediated gene silencing, production of miRNAs; mRNA catabolic process; phosphoinositide-mediated signaling; pre-microRNA processing; RNA secondary structure unwinding; RNA-mediated posttranscriptional gene silencing; Wnt receptor signaling pathway, calcium modulating pathway