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Western Blot (WB) (Western blot analysis of ARGLU1 Antibody (N-term) in A549, HL-60, 293, Jurkat cell line lysates (35ug/lane). ARGLU1 (arrow) was detected using the purified Pab.)

Rabbit ARGLU1 Polyclonal Antibody | anti-ARGLU1 antibody

ARGLU1 Antibody (N-term)

Reactivity
Human (Predicted Reactivity: Bovine)
Applications
ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
ARGLU1; Polyclonal Antibody; ARGLU1 Antibody (N-term); Arginine and glutamate-rich protein 1; anti-ARGLU1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This ARGLU1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 48-74 amino acids from the N-terminal region of human ARGLU1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.36 (varies by lot)
Sequence Positions
48-74
Sequence Length
198
Applicable Applications for anti-ARGLU1 antibody
ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Western Blot (WB)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of ARGLU1 Antibody (N-term) in A549, HL-60, 293, Jurkat cell line lysates (35ug/lane). ARGLU1 (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of ARGLU1 Antibody (N-term) in A549, HL-60, 293, Jurkat cell line lysates (35ug/lane). ARGLU1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human testis tissue reacted with ARGLU1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human testis tissue reacted with ARGLU1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(ARGLU1 Antibody (N-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (ARGLU1 Antibody (N-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-ARGLU1 antibody
ARGLU1 belongs to the UPF0430 family. There are two named isoforms.
Product Categories/Family for anti-ARGLU1 antibody
References
Olsen,J.V., et.al., Cell 127 (3), 635-648 (2006)
Beausoleil,S.A., et.al., Proc. Natl. Acad. Sci. U.S.A. 101 (33), 12130-12135 (2004)
Beausoleil,S.A., et.al., Proc. Natl. Acad. Sci. U.S.A. 101 (33), 12130-12135 (2004)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
33216
NCBI Official Full Name
arginine and glutamate-rich protein 1
NCBI Official Synonym Full Names
arginine and glutamate rich 1
NCBI Official Symbol
ARGLU1
NCBI Protein Information
arginine and glutamate-rich protein 1
UniProt Protein Name
Arginine and glutamate-rich protein 1
UniProt Gene Name
ARGLU1
UniProt Entry Name
ARGL1_HUMAN

Uniprot Description

ARGLU1: Required for the estrogen-dependent expression of ESR1 target genes. Can act in cooperation with MED1. Belongs to the UPF0430 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 13q33.3

Cellular Component: nucleoplasm; mitochondrion

Molecular Function: protein binding

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent

Research Articles on ARGLU1

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Product Notes

The ARGLU1 arglu1 (Catalog #AAA9212256) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 48-74. The ARGLU1 Antibody (N-term) reacts with Human (Predicted Reactivity: Bovine) and may cross-react with other species as described in the data sheet. AAA Biotech's ARGLU1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Western Blot (WB). WB~~1:1000. Researchers should empirically determine the suitability of the ARGLU1 arglu1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ARGLU1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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