Rabbit anti-Human ARGBP2 Polyclonal Antibody | anti-ARGBP2 antibody

ARGBP2, NT (Sorbin and SH3 Domain-containing Protein 2, Arg/Abl-interacting Protein 2, ArgBP2, Sorbin, KIAA0777, SORBS2) (PE)

Cat. Num. AAA6463921

• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: ARGBP2; Polyclonal Antibody; NT (Sorbin and SH3 Domain-containing Protein 2; Arg/Abl-interacting Protein 2; ArgBP2; Sorbin; KIAA0777; SORBS2) (PE); anti-ARGBP2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ARGBP2.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
• Sequence Length: 1100

• Applicable Applications for anti-ARGBP2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human ARGBP2 (KLH).
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Immunofluorescence (IF)

(Fluorescent image of U251 cell stained with ARGBP2 Antibody (N-term).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ARGBP2 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C).ARGBP2 immunoreactivity is localized to Nucleus significantly. )

Western Blot (WB)

(Western blot analysis of ARGBP2 Antibody (N-term) in MDA-MB231 cell line lysates (35ug/lane). ARGBP2 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human colon carcinoma reacted with ARGBP2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of MDA-231 cells using ARGBP2 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-ARGBP2 antibody

Antibodies; Tyrosine Kinases

References

1. Yuan, Z.Q., et.al., J. Biol. Chem. 280(22), 21483-21490 (2005). 2. Olsen, J.V., et.al., Cell 127(3), 635-648 (2006). 3. Taieb, D., et.al., Cancer Res. 68(12), 4588-4596 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 10947118
• NCBI Accession #: NP_066547
• NCBI GenBank Nucleotide #: NP_066547.1
• UniProt Accession #: O94875
• Molecular Weight: 124.108
• NCBI Official Full Name: sorbin and SH3 domain-containing protein 2 isoform 2
• UniProt Protein Name: Sorbin and SH3 domain-containing protein 2
• UniProt Gene Name: SORBS2
• UniProt Synonym Gene Names: ARGBP2; KIAA0777; ArgBP2
• UniProt Entry Name: SRBS2_HUMAN

Uniprot Description

ArgBP2: Adapter protein that plays a role in the assembling of signaling complexes, being a link between ABL kinases and actin cytoskeleton. Can form complex with ABL1 and CBL, thus promoting ubiquitination and degradation of ABL1 or with AKT1 and PAK1, thus mediating AKT1-mediated activation of PAK1. Isoform 6 increases water and sodium absorption in the intestine and gall-bladder. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 4q35.1

Cellular Component: perinuclear region of cytoplasm; plasma membrane; nucleus; Z disc; actin cytoskeleton

Molecular Function: protein domain specific binding; protein binding; structural constituent of cytoskeleton; structural constituent of muscle; metal ion binding; cytoskeletal adaptor activity

Biological Process: cell migration; actin filament organization; cell adhesion