Rabbit ARFGAP1 Polyclonal Antibody | anti-ARFGAP1 antibody

ARFGAP1 Rabbit Polyclonal

Cat. Num. AAA766768

• Gene Names: ARFGAP1; ARF1GAP; HRIHFB2281
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunoprecipitation
• Purity: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Synonyms: ARFGAP1; Polyclonal Antibody; ARFGAP1 Rabbit Polyclonal; anti-ARFGAP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 293

• Applicable Applications for anti-ARFGAP1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
• Immunogen: ADP-ribosylation factor GTPase activating protein 1
• Preparation and Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat kidney using MBS766768 (ARFGAP1 antibody) at dilution of 1:100)

Western Blot (WB)

(PC-3 cells were subjected to SDS PAGE followed by western blot with MBS766768 (ARFGAP1 antibody) at dilution of 1:1000)

NCBI and Uniprot Product Information

• NCBI GI #: 527498258
• NCBI GeneID: 55738
• NCBI Accession #: NP_001268413.1
• NCBI GenBank Nucleotide #: NM_001281484.1
• Molecular Weight: 39,705 Da
• NCBI Official Full Name: ADP-ribosylation factor GTPase-activating protein 1 isoform e
• NCBI Official Synonym Full Names: ADP ribosylation factor GTPase activating protein 1
• NCBI Official Symbol: ARFGAP1
• NCBI Official Synonym Symbols: ARF1GAP; HRIHFB2281
• NCBI Protein Information: ADP-ribosylation factor GTPase-activating protein 1
• UniProt Protein Name: ADP-ribosylation factor GTPase-activating protein 1
• Protein Family: ADP-ribosylation factor GTPase-activating protein
• UniProt Gene Name: ARFGAP1
• UniProt Synonym Gene Names: ARF1GAP; ARF GAP 1; ARF1 GAP
• UniProt Entry Name: ARFG1_HUMAN

NCBI Description

The protein encoded by this gene is a GTPase-activating protein, which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

ARF GAP1: GTPase-activating protein (GAP) for the ADP ribosylation factor 1 (ARF1). Involved in membrane trafficking and /or vesicle transport. Promotes hydrolysis of the ARF1-bound GTP and thus, is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles, a prerequisite for vesicle's fusion with target compartment. Probably regulates ARF1-mediated transport via its interaction with the KDELR proteins and TMED2. Overexpression induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, as when ARF1 is deactivated. Its activity is stimulated by phosphoinosides and inhibited by phosphatidylcholine. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; GAPs; GAPs, ARF

Chromosomal Location of Human Ortholog: 20q13.33

Cellular Component: cytosol; Golgi membrane

Molecular Function: GTPase activator activity; metal ion binding; protein binding

Biological Process: ER to Golgi vesicle-mediated transport; positive regulation of GTPase activity; protein transport; regulation of endocytosis; retrograde vesicle-mediated transport, Golgi to ER