Immunohistochemistry (IHC)
(Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE))
Rabbit anti-Mouse, Human ARF6 Polyclonal Antibody | anti-ARF6 antibody
Anti-ARF6 Antibody (aa84-113) IHC-plus
IHC-P (5 ug/ml)
WB (1:1000)
For long term storage store at -20 degree C.
Immunohistochemistry (IHC)
(Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE))
Immunohistochemistry (IHC)
(Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE))
Western Blot (WB)
(Western blot of ARF6 Antibody (Center) in T47D,MCF-7 cell line and mouse bladder tissue lysates (35 ug/lane). ARF6 (arrow) was detected using the purified Pab.(2 ug/ml))
Western Blot (WB)
(Western blot of ARF6 Antibody (Center) in T47D,MCF-7 cell line and mouse bladder tissue lysates (35 ug/lane). ARF6 (arrow) was detected using the purified Pab.(2 ug/ml))
Western Blot (WB)
(Western blot of ARF6 (arrow) using rabbit polyclonal ARF6 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ARF6 gene (Lane 2).)
Western Blot (WB)
(Western blot of ARF6 (arrow) using rabbit polyclonal ARF6 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ARF6 gene (Lane 2).)
Flow Cytometry (FC/FACS)
(ARF6 Antibody (Center) flow cytometry of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(ARF6 Antibody (Center) flow cytometry of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the human ARF gene family, which is part of the RAS superfamily. The ARF genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The product of this gene is localized to the plasma membrane, and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling. A pseudogene of this gene is located on chromosome 7. [provided by RefSeq, Jul 2008]
Uniprot Description
ARF6: GTP-binding protein involved in protein trafficking; regulates endocytic recycling and cytoskeleton remodeling. May modulate vesicle budding and uncoating within the Golgi apparatus. Functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in the regulation of dendritic spine development. Contributes to the regulation of dendritic branching and filopodia extension. Interacts with ARHGAP21, ASAP2, HERC1, PIP5K1C and UACA. Interacts with NCS1/FREQ at the plasma membrane. Interacts with RAB11FIP3 and RAB11FIP4. Interacts with USP6 (via Rab-GAP TBC domain). Interacts with ECM29. Interacts with TBC1D24. Belongs to the small GTPase superfamily. Arf family.
Protein type: G protein, monomeric; Motility/polarity/chemotaxis; G protein, monomeric, ARF
Chromosomal Location of Human Ortholog: 14q21.3
Cellular Component: cell cortex; cleavage furrow; early endosome; endocytic vesicle; endosome; filopodium membrane; focal adhesion; Golgi apparatus; membrane; midbody; myelin sheath; plasma membrane; recycling endosome membrane; ruffle
Molecular Function: GTP binding; GTPase activity; protein binding; protein N-terminus binding; thioesterase binding
Biological Process: cell adhesion; cell cycle; cell division; cell motility; cortical actin cytoskeleton organization and biogenesis; liver development; myeloid cell apoptosis; negative regulation of receptor-mediated endocytosis; positive regulation of actin filament polymerization; protein transport; regulation of filopodium formation; regulation of Rac protein signal transduction; ruffle organization and biogenesis; small GTPase mediated signal transduction; vesicle-mediated transport
Research Articles on ARF6
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Product Notes
The ARF6 arf6 (Catalog #AAA2400434) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-ARF6 Antibody (aa84-113) IHC-plus reacts with Mouse, Human and may cross-react with other species as described in the data sheet. AAA Biotech's ARF6 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS). Flow Cytometry (1:10 - 1:50) IHC-P (5 ug/ml) WB (1:1000). Researchers should empirically determine the suitability of the ARF6 arf6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ARF6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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