Rabbit anti-Rat TSC2 Polyclonal Antibody | anti-TSC2 antibody

TSC2, Phosphorylated (T1373) (Tsc2, Tuberin, Tuberous sclerosis 2 protein homolog) (FITC)

Cat. Num. AAA6359389

• Gene Names: Tsc2; Rc
• Reactivity: Rat
• Applications: Dot Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TSC2; Polyclonal Antibody; Phosphorylated (T1373) (Tsc2; Tuberin; Tuberous sclerosis 2 protein homolog) (FITC); Tsc2; Tuberous sclerosis 2 protein homolog; anti-TSC2 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-TSC2 antibody: FLISA, Dot Blot (DB)
• Application Notes: FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Rat TSC2 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T1373 of rat TSC2.
• Conjugate: FITC
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Dot Blot (DB)

(Dot blot analysis of rat TSC2 Antibody (Phospho T1373) Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)

Related Product Information for anti-TSC2 antibody

Acts as a tumor suppressor; may play a role in cell cycle regulation; acute phase response, and negative regulation of cell proliferation [RGD].

Product Categories/Family for anti-TSC2 antibody

Antibodies; Disease Markers

References

Larson, Y., et al. J. Biol. Chem. 285(32):24987-24998(2010). Sajankila, S.P., et al. Mol. Cell. Biochem. 338 (1-2), 233-239 (2010): Inoue, H., et al. Biosci. Biotechnol. Biochem. 73(11):2488-2493(2009). Di Nardo, A., et al. J. Neurosci. 29(18):5926-5937(2009). Shiono, M., et al. Oncogene 27(52):6690-6697(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 31543896
• NCBI GeneID: 24855
• NCBI Accession #: NP_036812.2
• NCBI GenBank Nucleotide #: NM_012680.3
• UniProt Accession #: P49816
• Molecular Weight: 194,006 Da
• NCBI Official Full Name: tuberin
• NCBI Official Synonym Full Names: tuberous sclerosis 2
• NCBI Official Symbol: Tsc2
• NCBI Official Synonym Symbols: Rc
• NCBI Protein Information: tuberin
• UniProt Protein Name: Tuberin
• Protein Family: Tuberin
• UniProt Gene Name: Tsc2
• UniProt Entry Name: TSC2_RAT

NCBI Description

acts as a tumor suppressor; may play a role in cell cycle regulation; acute phase response, and negative regulation of cell proliferation [RGD, Feb 2006]

Uniprot Description

In complex with TSC1, this tumor suppressor inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. Acts as a GTPase-activating protein (GAP) for the small GTPase RHEB, a direct activator of the protein kinase activity of mTORC1. May also play a role in microtubule-mediated protein transport. Also stimulates the intrinsic GTPase activity of the Ras-related proteins RAP1A and RAB5.