Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using ARF1 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 60s.)
Rabbit ARF1 Polyclonal Antibody | anti-ARF1 antibody
ARF1
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using ARF1 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 60s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using ARF1 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 60s.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded mouse brain using ARF1 antibody (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded mouse brain using ARF1 antibody (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded rat kidney using ARF1 antibody (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded rat kidney using ARF1 antibody (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human prostate using ARF1 antibody (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human prostate using ARF1 antibody (40x lens).)
NCBI and Uniprot Product Information
NCBI Description
ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
ARF1: GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP- ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles. Belongs to the small GTPase superfamily. Arf family.
Protein type: G protein; G protein, monomeric, ARF; Motility/polarity/chemotaxis; G protein, monomeric
Chromosomal Location of Human Ortholog: 1q42
Cellular Component: Golgi membrane; postsynaptic membrane; neuron projection; sarcomere; focal adhesion; perinuclear region of cytoplasm; postsynaptic density; plasma membrane; cytosol
Molecular Function: GTPase activity; protein binding; GTP binding; receptor signaling protein activity
Biological Process: protein transport; cellular copper ion homeostasis; COPI coating of Golgi vesicle; viral reproduction; regulation of receptor internalization; small GTPase mediated signal transduction; phospholipid metabolic process; phosphatidylinositol biosynthetic process; retrograde vesicle-mediated transport, Golgi to ER; antigen processing and presentation of exogenous peptide antigen via MHC class II; post-Golgi vesicle-mediated transport; regulation of defense response to virus by virus
Research Articles on ARF1
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Product Notes
The ARF1 arf1 (Catalog #AAA127076) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ARF1 reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ARF1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Researchers should empirically determine the suitability of the ARF1 arf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ARF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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