Rabbit anti-Human AR Polyclonal Antibody | anti-AR antibody

AR (Phospho-S213) Polyclonal Antibody

Cat. Num. AAA3004504

• Gene Names: AR; KD; AIS; AR8; TFM; DHTR; SBMA; HYSP1; NR3C4; SMAX1; HUMARA
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: AR; Polyclonal Antibody; AR (Phospho-S213) Polyclonal Antibody; Androgen receptor; Dihydrotestosterone receptor; Nuclear receptor subfamily 3 group C member 4; DHTR; NR3C4; anti-AR antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: p-AR (S213) polyclonal antibody detects endogenous levels of Androgen Receptor protein only when phosphorylated at Ser213.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 920

• Applicable Applications for anti-AR antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:50-1:200
• Immunogen: Synthetic phosphopeptide derived from human AR around the phosphorylation site of Serine 213.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of p-AR (S213) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)

Related Product Information for anti-AR antibody

Androgens exhibit a wide range of effects on the development, maintenance and regulation of male phenotype and reproductive physiology in males. The androgen receptor (AR) is a member of the steroid superfamily of ligand-dependent transcription factors. ARs bind active testosterone (T) and dihydrotestosterone (DHT). The rates of association and dissociation of T are about 3 times more rapid than those of DHT. This difference in binding kinetics may account for the different physiological effects of T and DHT. Androgen binding results in an at least 6-fold increase in androgen receptor stability.

NCBI and Uniprot Product Information

• NCBI GI #: 21322252
• NCBI GeneID: 367
• NCBI Accession #: NP_000035.2
• NCBI GenBank Nucleotide #: NP_000035.2
• UniProt Accession #: P10275
• Molecular Weight: ~ 110kDa
• NCBI Official Full Name: androgen receptor isoform 1
• NCBI Official Synonym Full Names: androgen receptor
• NCBI Official Symbol: AR
• NCBI Official Synonym Symbols: KD; AIS; AR8; TFM; DHTR; SBMA; HYSP1; NR3C4; SMAX1; HUMARA
• NCBI Protein Information: androgen receptor
• UniProt Protein Name: Androgen receptor
• Protein Family: Arpin
• UniProt Gene Name: AR
• UniProt Synonym Gene Names: DHTR; NR3C4
• UniProt Entry Name: ANDR_HUMAN

NCBI Description

The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract from the normal 9-34 repeats to the pathogenic 38-62 repeats causes spinal bulbar muscular atrophy (SBMA, also known as Kennedy's disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2017]

Uniprot Description

AR: a nuclear hormone receptor and transcription factor. Regulates gene expression and affects cellular proliferation and differentiation in target tissues. Two splice-variant isoforms have been described.

Protein type: Nuclear receptor; DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: Xq12

Cellular Component: nucleoplasm; protein complex; nuclear chromatin; cytoplasm; nucleus

Molecular Function: protein dimerization activity; ligand-dependent nuclear receptor activity; zinc ion binding; androgen binding; beta-catenin binding; transcription factor binding; protein binding; androgen receptor activity; enzyme binding; DNA binding; chromatin binding; transcription factor activity; receptor binding

Biological Process: prostate gland development; transcription initiation from RNA polymerase II promoter; intracellular receptor-mediated signaling pathway; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; signal transduction; protein oligomerization; activation of NF-kappaB transcription factor; negative regulation of integrin biosynthetic process; cell proliferation; cell-cell signaling; transport; positive regulation of cell proliferation; androgen receptor signaling pathway; positive regulation of transcription from RNA polymerase III promoter; gene expression; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling; positive regulation of integrin biosynthetic process; cell growth; sex differentiation; positive regulation of phosphorylation

Disease: Androgen Insensitivity Syndrome; Prostate Cancer; Androgen Insensitivity, Partial; Hypospadias 1, X-linked; Spinal And Bulbar Muscular Atrophy, X-linked 1