Chicken anti-Rat Aquaporin 8 Polyclonal Antibody | anti-AQP8 antibody
Aquaporin 8 (AQP8)
Gene Names
AQP8; AQP-8
Reactivity
Rat
Applications
ELISA, Western Blot, Immunoprecipitation
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
Aquaporin 8; Polyclonal Antibody; Aquaporin 8 (AQP8); Anti -Aquaporin 8 (AQP8); anti-AQP8 antibody
Host
Chicken
Reactivity
Rat
Clonality
Polyclonal
Isotype
IgY
Specificity
Recognizes rat Aquaporin-8. The peptide is unique to Aquaporin-8 without significant homology to any other Aquaporins. Species Sequence Homology: mouse, 93%; human, 81%.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, 0.1% BSA.
Applicable Applications for anti-AQP8 antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)
Application Notes
Suitable for use in ELISA, Western Blot.
Dilution: ELISA: 0.5-1.0ug/ml
Western Blot: 1-10ug/ml (ECL). Use 0.5-1% milk for primary/secondary antibody enzyme conjugate incubations to suppress non-specific bands.
Optimal dilutions to be determined by researcher.
Dilution: ELISA: 0.5-1.0ug/ml
Western Blot: 1-10ug/ml (ECL). Use 0.5-1% milk for primary/secondary antibody enzyme conjugate incubations to suppress non-specific bands.
Optimal dilutions to be determined by researcher.
Immunogen
16aa synthetic peptide within the C-terminal, cytoplasmic domain of rat Aquaporin-8 (1).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Related Product Information for anti-AQP8 antibody
Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein "aquaporin." A new water channel, AQP8, has been identified in rat pancreas and testis by homology cloning (1). AQP8 (263aa; 28kD) is also found in liver, colon and salivary glands. AQP8 is expressed in all stages of spermatogenesis. Unlike other AQP, AQP8 has unusually long N-terminus and a short C-terminus. AQP families of proteins are predicted to contain six trans- membrane domains. The N and C-termini are predicted to be cytoplasmic. AQP8 has significant homology to various AQPs: g-TIP (plant water channel, 37%); AQP2 and MIP (37%), AQP1, AQP4, and AQP5 (30-34%); AQP3 (26%). AQP8 does not facilitate glycerol transport. APQ8 forms a mercury-sensitive water-specific channel. It may have an important role in spermatogenesis, in fertilization, and in the secretion of pancreatic juice and saliva.
Product Categories/Family for anti-AQP8 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
27,381 Da
NCBI Official Full Name
aquaporin 8
NCBI Official Synonym Full Names
aquaporin 8
NCBI Official Symbol
AQP8
NCBI Official Synonym Symbols
AQP-8
NCBI Protein Information
aquaporin-8; OTTHUMP00000122504
UniProt Protein Name
Aquaporin-8
Protein Family
UniProt Gene Name
AQP8
UniProt Entry Name
AQP8_HUMAN
NCBI Description
Aquaporin 8 (AQP8) is a water channel protein. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). Aquaporin 8 mRNA is found in pancreas and colon but not other tissues. [provided by RefSeq]
Uniprot Description
AQP8: Forms a water-specific channel; mercury-sensitive. Not permeable to glycerol or urea. Belongs to the MIP/aquaporin (TC 1.A.8) family.
Protein type: Membrane protein, multi-pass; Transporter; Transporter, aquaporin family; Membrane protein, integral; Channel, misc.
Chromosomal Location of Human Ortholog: 16p12
Cellular Component: intracellular canaliculus; intracellular membrane-bound organelle; integral to plasma membrane; apical part of cell; plasma membrane
Molecular Function: water channel activity; glycerol channel activity
Biological Process: glycerol transport; response to reactive oxygen species; cellular water homeostasis; transport; canalicular bile acid transport; water transport; transmembrane transport
Research Articles on AQP8
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Product Notes
The AQP8 aqp8 (Catalog #AAA623224) is an Antibody produced from Chicken and is intended for research purposes only. The product is available for immediate purchase. The Aquaporin 8 (AQP8) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Aquaporin 8 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP). Suitable for use in ELISA, Western Blot. Dilution: ELISA: 0.5-1.0ug/ml Western Blot: 1-10ug/ml (ECL). Use 0.5-1% milk for primary/secondary antibody enzyme conjugate incubations to suppress non-specific bands. Optimal dilutions to be determined by researcher. Researchers should empirically determine the suitability of the AQP8 aqp8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Aquaporin 8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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