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Western Blot (WB)

Rabbit Aquaporin 4 Polyclonal Antibody | anti-AQP4 antibody

Aquaporin 4 Antibody

Gene Names
AQP4; MIWC; WCH4
Reactivity
Bovine, Human, Mouse, Rat, Sheep
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Synonyms
Aquaporin 4; Polyclonal Antibody; Aquaporin 4 Antibody; AQP 4; Aquaporin type 4; Aquaporin-4; HMIWC2; Mercurial insensitive water channel; MGC22454; MIWC; WCH 4 antibody; anti-AQP4 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Bovine, Human, Mouse, Rat, Sheep
Clonality
Polyclonal
Form/Format
Affinity Purified
Concentration
0.50-0.62 ug/ul in antibody stabilization buffer (varies by lot)
Sequence Length
323
Applicable Applications for anti-AQP4 antibody
ELISA (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
Application Notes
ELISA: 1:10,000
Immunocytochemistry: 1:100
Immunofluorescence: 1:100
Immunohistochemistry: 1:100
Immunoprecipitation: 1:200
Western Blot: 1:500
Immunogen
Synthetic peptide taken within amino acid region 300-323 on rat Aquaporin 4 protein.
Immunoglobulin
IgG
Expression
Abundant in mature brain but only weakly detectable in eye, kidney, intestine, and lung.
Molecular Function
Transport
Structure
Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
Subcellular Location
Membrane; Multi-pass protein
Preparation and Storage
-20 degree C for long term storage

Western Blot (WB)

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (IHC)
Related Product Information for anti-AQP4 antibody
Forms water specific channel. Osmoreceptor which regulates water balance and mediates water flow within CNS.
Product Categories/Family for anti-AQP4 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
361
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
32,300 Da
NCBI Official Full Name
aquaporin-4 isoform M1
NCBI Official Synonym Full Names
aquaporin 4
NCBI Official Symbol
AQP4
NCBI Official Synonym Symbols
MIWC; WCH4
NCBI Protein Information
aquaporin-4
UniProt Protein Name
Aquaporin-4
Protein Family
UniProt Gene Name
AQP4
UniProt Synonym Gene Names
AQP-4; MIWC
UniProt Entry Name
AQP4_HUMAN

NCBI Description

This gene encodes a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. This protein is the predominant aquaporin found in brain and has an important role in brain water homeostasis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. A recent study provided evidence for translational readthrough in this gene and expression of an additional C-terminally extended isoform via the use of an alternative in-frame translation termination codon. [provided by RefSeq, Dec 2015]

Uniprot Description

AQP4: a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. The predominant aquaporin found in brain. Two alternatively spliced transcript variants have been described

Protein type: Membrane protein, multi-pass; Channel, misc.; Transporter; Membrane protein, integral; Transporter, aquaporin family

Chromosomal Location of Human Ortholog: 18q11.2-q12.1

Cellular Component: basolateral plasma membrane; cytoplasm; external side of plasma membrane; integral to plasma membrane; plasma membrane

Molecular Function: glycerol channel activity; water channel activity; water transporter activity

Biological Process: cellular water homeostasis; glycerol transport; multicellular organismal water homeostasis; renal water homeostasis; transport; water transport

Research Articles on AQP4

Similar Products

Product Notes

The AQP4 aqp4 (Catalog #AAA540329) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Aquaporin 4 Antibody reacts with Bovine, Human, Mouse, Rat, Sheep and may cross-react with other species as described in the data sheet. AAA Biotech's Aquaporin 4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB). ELISA: 1:10,000 Immunocytochemistry: 1:100 Immunofluorescence: 1:100 Immunohistochemistry: 1:100 Immunoprecipitation: 1:200 Western Blot: 1:500. Researchers should empirically determine the suitability of the AQP4 aqp4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Aquaporin 4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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