Rabbit anti-Human Aquaporin 1 Polyclonal Antibody | anti-AQP1 antibody

Anti-Aquaporin 1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8101732

• Gene Names: AQP1; CO; CHIP28; AQP-CHIP
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: Aquaporin 1; Polyclonal Antibody; Anti-Aquaporin 1 Antibody; Rabbit Polyclonal; Aquaporin 1/AQP1 Antibody; Rabbit PAb; Antigen Affinity Purified; aquaporin 1 (Colton blood group); Anti-AQP-CHIP Antibody; Anti-CHIP28 Antibody; Anti-CO Antibody; anti-AQP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human Aquaporin 1/AQP1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-AQP1 antibody: Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: IHC-P: 1:500-1:2000
• Immunogen: A synthetic peptide corresponding to the C-terminus of the Human Aquaporin 1/AQP1
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human Aquaporin 1/AQP1, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human AQP1 in human kidney with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining of human AQP1 in human spleen with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

Related Product Information for anti-AQP1 antibody

Aquaporins (AQPs) are a family of hydrophobic integral membrane proteins that function as transmembrane channels and play an important role in tissue homeostasis. Aquaporin-1 (AQP1), in particular, has been reported to be involved in several biological processes including inflammation, angiogenesis, wound healing and others. Aquaporin-1 (AQP1) is expressed in the heart and it has been reported to transport nitric oxide (NO), an important regulator of cardiac function. AQP1 may be a useful identification and prognosis marker for osteosarcoma. AQP1 knockdown can effectively inhibit cell proliferation, adhesion, invasion and tumorigenesis by targeting TGF-beta signaling pathway and focal adhesion genes, which may serve a promising therapeutic strategy for osteosarcoma. AQP1 plays an important role in gastric adenocarcinoma and may therefore represent a novel therapeutic target and prognostic marker in this disease. Malignant pleural mesothelioma (MPM) is an aggressive cancer. MPM cells express aquaporin-1 (AQP1) that in other cancers has been shown to participate in the tumor metastasis processes. AQP1 is expressed in atherosclerotic lesion neovasculature in human and mouse arteries and AQP1 deficiency augments lesion development in ANGII-promoted atherosclerosis in mice. Normal function of AQP1 affords cardiovascular protection. AQP1 effectively downregulated AQP1 expression at the mRNA and protein levels, markedly suppressed cell viability, migration and invasion and promoted apoptosis of ovarian cancers cells. These results suggested that AQP1 may serve as a novel target for ovarian cancer treatment in the future.

NCBI and Uniprot Product Information

• NCBI GI #: 297307116
• NCBI GeneID: 358
• NCBI Accession #: NP_001171989.1
• NCBI GenBank Nucleotide #: NM_001185060.1
• UniProt Accession #: P29972; Q8TBI5; Q8TDC1; B5BU39; F5GY19
• Molecular Weight: 28,526 Da
• NCBI Official Full Name: aquaporin-1 isoform 2
• NCBI Official Synonym Full Names: aquaporin 1 (Colton blood group)
• NCBI Official Symbol: AQP1
• NCBI Official Synonym Symbols: CO; CHIP28; AQP-CHIP
• NCBI Protein Information: aquaporin-1; aquaporin-CHIP; Colton blood group; urine water channel; channel-like integral membrane protein, 28-kDa; water channel protein for red blood cells and kidney proximal tubule; aquaporin 1 (channel-forming integral protein, 28kDa, CO blood grou
• UniProt Protein Name: Aquaporin-1
• UniProt Gene Name: AQP1
• UniProt Synonym Gene Names: CHIP28; AQP-1
• UniProt Entry Name: AQP1_HUMAN

NCBI Description

Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). This gene encodes an aquaporin which functions as a molecular water channel protein. It is a homotetramer with 6 bilayer spanning domains and N-glycosylation sites. The protein physically resembles channel proteins and is abundant in erythrocytes and renal tubes. The gene encoding this aquaporin is a possible candidate for disorders involving imbalance in ocular fluid movement. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]

Uniprot Description

AQP1: Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Belongs to the MIP/aquaporin (TC 1.A.8) family.

Protein type: Transporter, aquaporin family; Membrane protein, integral; Transporter; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 7p14

Cellular Component: nuclear membrane; integral to plasma membrane; basolateral plasma membrane; brush border membrane; apical part of cell; apical plasma membrane; cytoplasm; plasma membrane; basal plasma membrane; nerve terminal; nucleus; sarcolemma; brush border

Molecular Function: protein binding; ephrin receptor binding; potassium channel activity; water transporter activity; intracellular cGMP activated cation channel activity; ammonium transmembrane transporter activity; glycerol transmembrane transporter activity; potassium ion transmembrane transporter activity; nitric oxide transporter activity; water channel activity; transmembrane transporter activity; glycerol channel activity

Biological Process: cGMP biosynthetic process; glycerol transport; cerebrospinal fluid secretion; wound healing; cellular response to stress; water transport; carbon dioxide transport; negative regulation of caspase activity; pancreatic juice secretion; sensory perception of pain; positive regulation of saliva secretion; odontogenesis; positive regulation of fibroblast proliferation; hyperosmotic salinity response; nitric oxide transport; bicarbonate transport; multicellular organismal water homeostasis; cellular homeostasis; transmembrane transport; potassium ion transport; lipid digestion; lateral ventricle development; response to drug; cellular water homeostasis; secretory granule organization and biogenesis; glomerular filtration; ammonium transport; renal water transport; positive regulation of angiogenesis; camera-type eye morphogenesis; adrenocorticotropin hormone secretion; response to estrogen stimulus; establishment and/or maintenance of actin cytoskeleton polarity; renal water homeostasis; cell volume homeostasis; negative regulation of apoptosis

Disease: Blood Group--colton