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Western Blot (WB) (Western blot analysis of extracts of various cell lines, using AQP4 antibody.)

Rabbit AQP4 Polyclonal Antibody | anti-AQP4 antibody

AQP4 (Aquaporin 4, MIWC, HMIWC2) (FITC)

Gene Names
AQP4; MIWC; HMIWC2
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Purified by affinity chromatography.
Synonyms
AQP4; Polyclonal Antibody; AQP4 (Aquaporin 4; MIWC; HMIWC2) (FITC); anti-AQP4 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes AQP4. Species Crossreactivity: human, mouse, rat
Purity/Purification
Purified by affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Concentration
~1mg/ml (varies by lot)
Applicable Applications for anti-AQP4 antibody
Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
IHC: 1:50-1:200
WB: 1:500-1:2000
Applications are based on unconjugated antibody.
Immunogen
Recombinant protein corresponding to human AQP4.
Conjugate
FITC
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using AQP4 antibody.)

Western Blot (WB) (Western blot analysis of extracts of various cell lines, using AQP4 antibody.)
Product Categories/Family for anti-AQP4 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
361
UniProt Accession #
Molecular Weight
323
NCBI Official Full Name
Aquaporin-4
NCBI Official Synonym Full Names
aquaporin 4
NCBI Official Symbol
AQP4
NCBI Official Synonym Symbols
MIWC; HMIWC2
NCBI Protein Information
aquaporin-4; WCH4; aquaporin type4; aquaporin 4 isoform delta4; mercurial-insensitive water channel
UniProt Protein Name
Aquaporin-4
Protein Family
UniProt Gene Name
AQP4
UniProt Synonym Gene Names
AQP-4; MIWC
UniProt Entry Name
AQP4_HUMAN

NCBI Description

This gene encodes a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. The encoded protein is the predominant aquaporin found in brain. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

AQP4: a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. The predominant aquaporin found in brain. Two alternatively spliced transcript variants have been described

Protein type: Transporter, aquaporin family; Transporter; Channel, misc.; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 18q11.2-q12.1

Cellular Component: protein complex; integral to plasma membrane; basolateral plasma membrane; cytoplasm; T-tubule; plasma membrane; intercellular junction; external side of plasma membrane

Molecular Function: water transporter activity; water channel activity; porin activity; glycerol channel activity

Biological Process: glycerol transport; cellular water homeostasis; carbon dioxide transport; water transport; response to glucocorticoid stimulus; female pregnancy; response to radiation; sensory perception of sound; hyperosmotic salinity response; transport; renal water homeostasis; multicellular organismal water homeostasis; transmembrane transport; protein homooligomerization

Research Articles on AQP4

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Product Notes

The AQP4 aqp4 (Catalog #AAA6406985) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AQP4 (Aquaporin 4, MIWC, HMIWC2) (FITC) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's AQP4 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Western Blot (WB). IHC: 1:50-1:200 WB: 1:500-1:2000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the AQP4 aqp4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AQP4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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