Rabbit anti-Human, Mouse AQP3 Polyclonal Antibody | anti-AQP3 antibody

AQP3, ID (AQP3, Aquaporin-3, Aquaglyceroporin-3) (FITC)

Cat. Num. AAA6274646

• Gene Names: AQP3; GIL; AQP-3
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: AQP3; Polyclonal Antibody; ID (AQP3; Aquaporin-3; Aquaglyceroporin-3) (FITC); Aquaglyceroporin-3; anti-AQP3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-AQP3 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: AQP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 163-191 amino acids from the Central region of human AQP3.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(AQP3 Antibody (Center) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the AQP3 antibody detected the AQP3 protein (arrow).)

Western Blot (WB)

(AQP3 Antibody (Center) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the AQP3 antibody detected the AQP3 protein (arrow).)

Related Product Information for anti-AQP3 antibody

Aquaporin 3 is a water channel protein. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). Aquaporin 3 is localized at the basal lateral membranes of collecting duct cells in the kidney. In addition to its water channel function, aquaporin 3 has been found to facilitate the transport of nonionic small solutes such as urea and glycerol, but to a smaller degree. It has been suggested that water channels can be functionally heterogeneous and possess water and solute permeation mechanisms.

Product Categories/Family for anti-AQP3 antibody

Antibodies; Aquaporin

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Kim, N.H., et al. J. Invest. Dermatol. 130(9):2231-2239(2010). Ji, C., et al. Int. J. Mol. Med. 26(2):257-263(2010). Melis, M., et al. Dis. Colon Rectum 53(6):936-943(2010). Shen, L., et al. Biomed. Pharmacother. 64(5):313-318(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 4826645
• NCBI GeneID: 360
• NCBI Accession #: NP_004916.1
• NCBI GenBank Nucleotide #: NM_004925.4
• UniProt Accession #: Q92482; O00108; Q6FGT2; Q6FGW6; A8K843; B2RE16; D3DRL3
• Molecular Weight: 31,544 Da
• NCBI Official Full Name: aquaporin-3
• NCBI Official Synonym Full Names: aquaporin 3 (Gill blood group)
• NCBI Official Symbol: AQP3
• NCBI Official Synonym Symbols: GIL; AQP-3
• NCBI Protein Information: aquaporin-3; aquaglyceroporin-3; aquaporin 3 (GIL blood group)
• UniProt Protein Name: Aquaporin-3
• Protein Family: Aquaporin
• UniProt Gene Name: AQP3
• UniProt Synonym Gene Names: AQP-3
• UniProt Entry Name: AQP3_HUMAN

NCBI Description

This gene encodes the water channel protein aquaporin 3. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein, also known as aquaporin 0. Aquaporin 3 is localized at the basal lateral membranes of collecting duct cells in the kidney. In addition to its water channel function, aquaporin 3 has been found to facilitate the transport of nonionic small solutes such as urea and glycerol, but to a smaller degree. It has been suggested that water channels can be functionally heterogeneous and possess water and solute permeation mechanisms. [provided by RefSeq, Aug 2011]

Uniprot Description

AQP3: Water channel required to promote glycerol permeability and water transport across cell membranes. Acts as a glycerol transporter in skin and plays an important role in regulating SC (stratum corneum) and epidermal glycerol content. Involved in skin hydration, wound healing, and tumorigenesis. Provides kidney medullary collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Slightly permeable to urea and may function as a water and urea exit mechanism in antidiuresis in collecting duct cells. It may play an important role in gastrointestinal tract water transport and in glycerol metabolism. Belongs to the MIP/aquaporin (TC 1.A.8) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Transporter, aquaporin family; Membrane protein, integral; Transporter; Channel, misc.

Chromosomal Location of Human Ortholog: 9p13

Cellular Component: integral to plasma membrane; basolateral plasma membrane; cytoplasm; plasma membrane; integral to membrane; intercellular junction

Molecular Function: transporter activity; urea transmembrane transporter activity; water channel activity; glycerol channel activity

Biological Process: odontogenesis; positive regulation of immune system process; glycerol transport; cellular water homeostasis; response to retinoic acid; response to vitamin D; transport; water transport; renal water homeostasis; response to calcium ion; excretion; regulation of keratinocyte differentiation; transmembrane transport

Disease: Gil Blood Group