Rabbit APTX Polyclonal Antibody | anti-APTX antibody

APTX (Aprataxin, AOA, AOA1, AXA1, EAOH, EOAHA, FHA-HIT) (FITC)

Cat. Num. AAA6406930

• Gene Names: APTX; AOA; AOA1; AXA1; EAOH; EOAHA; FHA-HIT
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: APTX; Polyclonal Antibody; APTX (Aprataxin; AOA; AOA1; AXA1; EAOH; EOAHA; FHA-HIT) (FITC); anti-APTX antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes APTX. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-APTX antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IHC: 1:50-1:200; WB: 1:500-1:2000; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human APTX.
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using APTX antibody.)

Product Categories/Family for anti-APTX antibody

Antibodies; Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 305410829
• NCBI GeneID: 54840
• NCBI Accession #: NP_001182177.1
• NCBI GenBank Nucleotide #: NM_001195248.1
• UniProt Accession #: Q7Z2E3; Q0P662; Q5T781; Q5T782; Q5T784; Q6JV81; Q6JV82; Q6JV85; Q7Z2F3; A8MTN4; D3DRK9; D3DRL0
• Molecular Weight: 21,389 Da
• NCBI Official Full Name: aprataxin isoform e
• NCBI Official Synonym Full Names: aprataxin
• NCBI Official Symbol: APTX
• NCBI Official Synonym Symbols: AOA; AOA1; AXA1; EAOH; EOAHA; FHA-HIT
• NCBI Protein Information: aprataxin; forkhead-associated domain histidine triad-like protein
• UniProt Protein Name: Aprataxin
• Protein Family: Aprataxin
• UniProt Gene Name: APTX
• UniProt Synonym Gene Names: AXA1; FHA-HIT
• UniProt Entry Name: APTX_HUMAN

NCBI Description

This gene encodes a member of the histidine triad (HIT) superfamily. The encoded protein may play a role in single-stranded DNA repair through its nucleotide-binding activity and its diadenosine polyphosphate hydrolase activity. Mutations in this gene have been associated with ataxia-ocular apraxia. Alternatively spliced transcript variants have been identified for this gene.[provided by RefSeq, Aug 2010]

Uniprot Description

APTX: DNA-binding protein involved in single-strand DNA break repair, double-strand DNA break repair and base excision repair. Resolves abortive DNA ligation intermediates formed either at base excision sites, or when DNA ligases attempt to repair non- ligatable breaks induced by reactive oxygen species. Catalyzes the release of adenylate groups covalently linked to 5'-phosphate termini, resulting in the production of 5'-phosphate termini that can be efficiently rejoined. Also able to hydrolyze adenosine 5'- monophosphoramidate (AMP-NH(2)) and diadenosine tetraphosphate (AppppA), but with lower catalytic activity. Defects in APTX are the cause of ataxia-oculomotor apraxia syndrome (AOA). AOA is an autosomal recessive syndrome characterized by early-onset cerebellar ataxia, oculomotor apraxia, early areflexia and late peripheral neuropathy. 13 isoforms of the human protein are produced by alternative splicing.

Protein type: C2H2-type zinc finger protein; EC 3.-.-.-; Phosphatase (non-protein); DNA repair, damage; RNA-binding; Nucleolus

Chromosomal Location of Human Ortholog: 9p13.3

Cellular Component: nucleoplasm; nuclear chromatin; cytoplasm; nucleolus; nucleus; chromatin

Molecular Function: protein binding; DNA 5'-adenosine monophosphate hydrolase activity; phosphoglycolate phosphatase activity; polynucleotide 3'-phosphatase activity; metal ion binding; double-stranded DNA binding; double-stranded RNA binding; damaged DNA binding; protein N-terminus binding; phosphoprotein binding; chromatin binding

Biological Process: single strand break repair; dephosphorylation; response to hydrogen peroxide; double-strand break repair; regulation of protein stability; DNA catabolic process, exonucleolytic; response to DNA damage stimulus; DNA ligation

Disease: Ataxia, Early-onset, With Oculomotor Apraxia And Hypoalbuminemia