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Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Rabbit anti-Mouse Apoptosis Associated Tyrosine Kinase (AATK) Polyclonal Antibody | anti-AATK antibody

Polyclonal Antibody to Apoptosis Associated Tyrosine Kinase (AATK)

Gene Names
Aatk; AATYK; aatyk1; mKIAA0641
Reactivity
Mouse
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Apoptosis Associated Tyrosine Kinase (AATK); Polyclonal Antibody; Polyclonal Antibody to Apoptosis Associated Tyrosine Kinase (AATK); anti-AATK antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against AATK. It has been selected for its ability to recognize AATK in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- YLKE IGHGWFGKVF LGEVHSGVSG TQVVVKELKV SASVQEQMQF LEEAQPYRAL QHSNLLQCLA QCAEVTPYLL VMEFCPLGDL KGYLRSCRVT ESMAPDPLTL QRMACEVACG VLHLHRHNYV HSDLALRNCL LTADLTVKVG DYGLSHCKYR EDYLVTADQL WVPLRWIAPE LVDEVHGNLL VVDQTKSSNV
Sequence Length
1317
Applicable Applications for anti-AATK antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant AATK (Tyr137~Gln386) expressed in E.coli.
Cross Reactivity
Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2042281
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin-embedded Kidney tissue))

Immunohistochemistry (IHC) (DAB staining on fromalin fixed paraffin-embedded Kidney tissue))

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Mouse Testis Tissue))

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Mouse Testis Tissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
127,544 Da
NCBI Official Full Name
serine/threonine-protein kinase LMTK1 isoform 2
NCBI Official Synonym Full Names
apoptosis-associated tyrosine kinase
NCBI Official Symbol
Aatk
NCBI Official Synonym Symbols
AATYK; aatyk1; mKIAA0641
NCBI Protein Information
serine/threonine-protein kinase LMTK1
UniProt Protein Name
Serine/threonine-protein kinase LMTK1
UniProt Gene Name
Aatk
UniProt Synonym Gene Names
Aatyk; Kiaa0641; Lmr1; Lmtk1; AATYK

Uniprot Description

Lmr1: a protein kinase kinase of the Lmr family. Predominantly expressed in the nervous system and is involved in neurite extension and apoptosis of cerebellar granule cells. Contains a tyrosine kinase domain at the N-terminal end and a proline-rich domain at the C-terminal end. May be necessary for the induction of growth arrest and/or apoptosis of myeloid cells induced by cytokine withdrawal, such as IL3, and during G-CSF-induced terminal differentiation of myeloblasts to granulocytes. Four alternatively spliced isoforms have been reported.

Protein type: EC 2.7.11.1; Kinase, protein; Lmr family; Membrane protein, integral; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group

Chromosomal Location of Human Ortholog: 11|11 E2

Cellular Component: axonal growth cone; cell projection; cytoplasm; endoplasmic reticulum; integral component of membrane; membrane; perinuclear region of cytoplasm; recycling endosome

Molecular Function: ATP binding; kinase activity; nucleotide binding; protein binding; protein kinase activity; protein serine/threonine kinase activity; protein tyrosine kinase activity; transferase activity

Biological Process: apoptosis; brain development; negative regulation of axon extension; neuron apoptosis; peptidyl-tyrosine autophosphorylation; phosphorylation; protein amino acid phosphorylation; protein autophosphorylation; Rab protein signal transduction

Research Articles on AATK

Similar Products

Product Notes

The AATK aatk (Catalog #AAA2013857) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Apoptosis Associated Tyrosine Kinase (AATK) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Apoptosis Associated Tyrosine Kinase (AATK) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the AATK aatk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- YLKE IGHGWFGKVF LGEVHSGVSG TQVVVKELKV SASVQEQMQF LEEAQPYRAL QHSNLLQCLA QCAEVTPYLL VMEFCPLGDL KGYLRSCRVT ESMAPDPLTL QRMACEVACG VLHLHRHNYV HSDLALRNCL LTADLTVKVG DYGLSHCKYR EDYLVTADQL WVPLRWIAPE LVDEVHGNLL VVDQTKSSNV. It is sometimes possible for the material contained within the vial of "Apoptosis Associated Tyrosine Kinase (AATK), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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