Western Blot (WB)
((1ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat Apolipoprotein L6 Polyclonal Antibody | anti-APOL6 antibody
Goat anti-apolipoprotein L6 Antibody
Gene Names
APOL6; APOLVI; APOL-VI
Reactivity
Tested: Human; Expected from sequence similarity: Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
Apolipoprotein L6; Polyclonal Antibody; Goat anti-apolipoprotein L6 Antibody; Goat Anti-APOL6 Antibody; APOL6; apolipoprotein L; 6; APOL-VI; DKFZp667M075; FLJ38562; FLJ90164; MGC57495; apolipoprotein L-VI; apolipoprotein L6; APOL6 antibody; 6 antibody; APOL-VI antibody; DKFZp667M075 antibody; FLJ38562 antibody; FLJ90164 antibody; MGC57495 antibody; apolipoprotein L-VI antibody; apolipoprotein L6 antibody; anti-APOL6 antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
QRDEDDAPLCED
Sequence Length
343
Applicable Applications for anti-APOL6 antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 8000.
Immunohistochemistry: In paraffin embedded Human Liver shows textured cytoplasm staining in hepatocytes. Recommended concentration, 5-10ug/ml.
Western Blot: Approx.38kDa band observed in Human Kidney lysates (calculated MW of 38.1kDa according to NP_085144.1). Recommended concentration: 1-3ug/ml.
Immunohistochemistry: In paraffin embedded Human Liver shows textured cytoplasm staining in hepatocytes. Recommended concentration, 5-10ug/ml.
Western Blot: Approx.38kDa band observed in Human Kidney lysates (calculated MW of 38.1kDa according to NP_085144.1). Recommended concentration: 1-3ug/ml.
Immunogen
Peptide with sequence QRDEDDAPLCED, from the internal region (near N Terminus) of the protein sequence according to NP_085144.1.
Epitope
Internal region (near N Terminus)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((1ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((1ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Immunohistochemistry (IHC)
((5ug/ml) staining of paraffin embedded Human Liver. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.)
Immunohistochemistry (IHC)
((5ug/ml) staining of paraffin embedded Human Liver. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
38,128 Da
NCBI Official Full Name
apolipoprotein L6
NCBI Official Synonym Full Names
apolipoprotein L6
NCBI Official Symbol
APOL6
NCBI Official Synonym Symbols
APOLVI; APOL-VI
NCBI Protein Information
apolipoprotein L6
UniProt Protein Name
Apolipoprotein L6
Protein Family
UniProt Gene Name
APOL6
UniProt Synonym Gene Names
ApoL-VI
UniProt Entry Name
APOL6_HUMAN
NCBI Description
This gene is a member of the apolipoprotein L gene family. The encoded protein is found in the cytoplasm, where it may affect the movement of lipids or allow the binding of lipids to organelles. [provided by RefSeq, Jul 2008]
Uniprot Description
APOL6: May affect the movement of lipids in the cytoplasm or allow the binding of lipids to organelles. Belongs to the apolipoprotein L family.
Protein type: Apoptosis
Chromosomal Location of Human Ortholog: 22q12.3
Cellular Component: cytoplasm; extracellular region
Molecular Function: lipid binding
Biological Process: lipid transport; lipoprotein metabolic process
Research Articles on APOL6
Similar Products
Product Notes
The APOL6 apol6 (Catalog #AAA421660) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-apolipoprotein L6 Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's Apolipoprotein L6 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 8000. Immunohistochemistry: In paraffin embedded Human Liver shows textured cytoplasm staining in hepatocytes. Recommended concentration, 5-10ug/ml. Western Blot: Approx.38kDa band observed in Human Kidney lysates (calculated MW of 38.1kDa according to NP_085144.1). Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the APOL6 apol6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: QRDEDDAPLC ED. It is sometimes possible for the material contained within the vial of "Apolipoprotein L6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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