Western Blot (WB)
((0.5ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat Apolipoprotein F Polyclonal Antibody | anti-APOF antibody
Goat anti-Apolipoprotein F Antibody
Gene Names
APOF; LTIP; Apo-F
Reactivity
Tested: Human; Expected from sequence similarity: Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
Apolipoprotein F; Polyclonal Antibody; Goat anti-Apolipoprotein F Antibody; APOF; Apo-F; DKFZp781G18150; MGC22520; Apolipoprotein F antibody; APOF antibody; Apo-F antibody; DKFZp781G18150 antibody; MGC22520 antibody; anti-APOF antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
KSYDLDPGAGSLE
Sequence Length
326
Applicable Applications for anti-APOF antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Immunohistochemistry: In paraffin embedded Human Liver shows staniing of droplets in the cytoplasm of hepatocytes. Recommended concentration, 2.5-3.8ug/ml.
Western Blot: Approx.35kDa band observed in Human Liver lysates (calculated MW of 35.4kDa according to NP_001629.1). Recommended concentration: 0.3-1ug/ml.
Immunohistochemistry: In paraffin embedded Human Liver shows staniing of droplets in the cytoplasm of hepatocytes. Recommended concentration, 2.5-3.8ug/ml.
Western Blot: Approx.35kDa band observed in Human Liver lysates (calculated MW of 35.4kDa according to NP_001629.1). Recommended concentration: 0.3-1ug/ml.
Immunogen
Peptide with sequence C-KSYDLDPGAGSLE, from the C Terminus of the protein sequence according to NP_001629.1.
Epitope
C Terminus
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((0.5ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.5ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Immunohistochemistry (IHC)
((3.8ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
((3.8ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35,399 Da
NCBI Official Full Name
apolipoprotein F
NCBI Official Synonym Full Names
apolipoprotein F
NCBI Official Symbol
APOF
NCBI Official Synonym Symbols
LTIP; Apo-F
NCBI Protein Information
apolipoprotein F
UniProt Protein Name
Apolipoprotein F
Protein Family
UniProt Gene Name
APOF
UniProt Synonym Gene Names
Apo-F; LTIP
UniProt Entry Name
APOF_HUMAN
NCBI Description
The product of this gene is one of the minor apolipoproteins found in plasma. This protein forms complexes with lipoproteins and may be involved in transport and/or esterification of cholesterol. [provided by RefSeq, Jul 2008]
Uniprot Description
APOF: Minor apolipoprotein that associates with LDL. Inhibits cholesteryl ester transfer protein (CETP) activity and appears to be an important regulator of cholesterol transport. Also associates to a lesser degree with VLDL, Apo-AI and Apo-AII.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 12q13.3
Cellular Component: extracellular region
Molecular Function: cholesterol binding; lipid transporter activity; receptor binding
Biological Process: cholesterol metabolic process; lipid metabolic process; lipid transport
Research Articles on APOF
Similar Products
Product Notes
The APOF apof (Catalog #AAA422185) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Apolipoprotein F Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's Apolipoprotein F can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 32000. Immunohistochemistry: In paraffin embedded Human Liver shows staniing of droplets in the cytoplasm of hepatocytes. Recommended concentration, 2.5-3.8ug/ml. Western Blot: Approx.35kDa band observed in Human Liver lysates (calculated MW of 35.4kDa according to NP_001629.1). Recommended concentration: 0.3-1ug/ml. Researchers should empirically determine the suitability of the APOF apof for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: KSYDLDPGAG SLE. It is sometimes possible for the material contained within the vial of "Apolipoprotein F, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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