Human Apolipoprotein D ELISA Kit | APOD elisa kit

Human Apolipoprotein D ELISA Kit

Cat. Num. AAA724117

• Reactivity: Human
• Synonyms: Apolipoprotein D; Human Apolipoprotein D ELISA Kit; APOD elisa kit

• Reactivity: Human

• Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate
• Assay Type: Quantitative Competitive or Sandwich
• Sensitivity: 0.1ug/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for APOD elisa kit

Intended Uses: This APOB ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat APOB. This ELISA kit for research use only!

Principle of the Assay: APOB ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for APOB. Standards or samples are then added to the microtiter plate wells and APOB if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of APOB present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for APOB are added to each well to "sandwich" the APOB immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain APOB and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The APOB concentration in each sample is interpolated from this standard curve.

Product Categories/Family for APOD elisa kit

Neurobiology

NCBI and Uniprot Product Information

• NCBI GI #: 4502163
• NCBI GeneID: 347
• NCBI Accession #: NP_001638.1
• NCBI GenBank Nucleotide #: NM_001647.3
• UniProt Accession #: P05090; Q6IBG6; B2R579; D3DNW6
• Molecular Weight: 21,276 Da
• NCBI Official Full Name: apolipoprotein D
• NCBI Official Synonym Full Names: apolipoprotein D
• NCBI Official Symbol: APOD
• NCBI Protein Information: apolipoprotein D; apo-D
• UniProt Protein Name: Apolipoprotein D
• Protein Family: Apolipoprotein
• UniProt Gene Name: APOD
• UniProt Synonym Gene Names: Apo-D; ApoD
• UniProt Entry Name: APOD_HUMAN

NCBI Description

This gene encodes a component of high density lipoprotein that has no marked similarity to other apolipoprotein sequences. It has a high degree of homology to plasma retinol-binding protein and other members of the alpha 2 microglobulin protein superfamily of carrier proteins, also known as lipocalins. This glycoprotein is closely associated with the enzyme lecithin:cholesterol acyltransferase - an enzyme involved in lipoprotein metabolism. [provided by RefSeq, Aug 2008]

Uniprot Description

Function: APOD occurs in the macromolecular complex with lecithin-cholesterol acyltransferase. It is probably involved in the transport and binding of bilin. Appears to be able to transport a variety of ligands in a number of different contexts.

Subunit structure: Homodimer. In plasma, also exists as a disulfide-linked heterodimer with APOA2. Ref.9

Subcellular location: Secreted.

Tissue specificity: Expressed in liver, intestine, pancreas, kidney, placenta, adrenal, spleen, fetal brain tissue and tears.

Post-translational modification: N-glycosylatd. N-glycan heterogeneity at Asn-65: Hex5HexNAc4 (major) and Hex6HexNAc5 (minor); at Asn-98: Hex5HexNAc4 (minor), dHex1Hex5HexNAc4 (major), dHex1Hex6HexNAc5 (minor) and dHex1Hex7HexNAc6 (minor).

Miscellaneous: APOD is primarily localized in HDL (60-65%), with most of the remainder in VHDL and only trace amounts in VLDL and LDL.

Sequence similarities: Belongs to the calycin superfamily. Lipocalin family.