Goat anti-Human Apolipoprotein B48/100 Polyclonal Antibody | anti-APOB antibody

Apolipoprotein B48/100 (Apo B48/100) (Biotin)

Cat. Num. AAA614884

• Gene Names: APOB; FLDB; LDLCQ4
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Apolipoprotein B48/100; Polyclonal Antibody; Apolipoprotein B48/100 (Apo B48/100) (Biotin); Anti -Apolipoprotein B48/100 (Apo B48/100) (Biotin); anti-APOB antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Apo B48 and B100. Does not crossreact with human Apo AI, Apo AII, Apo CI, Apo CII, Apo CIII or Apo E.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide. Labeled with Biotin.

• Applicable Applications for anti-APOB antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Radial Immunodiffusion, ELISA and Western Blot.; Dilution: ELISA: 1:4000-1:40,000.
• Immunogen: Human LDL.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-APOB antibody

Lipids (triglyceride (TG), cholesterol (Chol), phospholipids (PL), cholesteryl esters (CE)) are insoluble in plasma. These lipids combine with apolipoproteins to form lipoproteins. This process solubilises lipids in plasma. Lipoproteins provide a transport system for lipids. They also play an important role in lipoprotein receptor recognition and regulation of certain enzymes in lipoprotein metabolism. Apolipoprotein B plays an essential role in lipid transport and metabolism. Apo B may regulate cholesterol synthesis through its interaction with specific cell membrane receptors and by inhibition of HMG Co A reductase. This enzyme has been identified as the rate controlling enzyme in cholesterol biosynthesis. Apo B may be important in the genesis of atherosclerosis. Apo B is the major protein moiety of all lipoproteins other than high density lipoprotein (HDL) in plasma. Increased levels of apo B100 are associated with an increased risk of coronary artery disease. Apo B exists in human plasma as two isoforms: apo B48 and apo B100. Apo B100 is the major physiological ligand for the LDL receptor. It contains 4536 amino acid residues (Chen et al., 1986; Law et al., 1986). Apo B100 is the full length protein. Its gene has been mapped on the short arm of chromosome 2, with an approximate length of 43kb and 29 exons (Ludwig, et al., 1987). The LDL-binding domain of the molecule is proposed to be located between the aa3129-3532 (Knott, et al., 1986). Apo B100 is synthesized in the liver. It is required for the assembly of very low density lipoproteins (VLDL). It does not interchange between lipoprotein particles, as do the other lipoproteins. It is found in IDL and LDL particles after the removal of the apolipoproteins A, E and C (Young, 1990). Apo B48 is present in chylomicrons and chylomicron remnants. It plays an essential role in the intestinal absorption of dietary fats (Kane, 1983). Apo B48 is synthesized in the small intestine. It comprises the N-terminal 48% of apo B100.Apo B48 is the degraded protein. Apo B48 lacks the LDL receptor-binding domain of Apo B100. It is produced due to post-transscriptional Apo B100 mRNA editing at codon 2153, which creates a stop codon in the intestine instead of a glutamine in the liver (Chen et al., 1987).

Product Categories/Family for anti-APOB antibody

Antibodies; Abs to Lipoproteins

NCBI and Uniprot Product Information

• NCBI GI #: 105990532
• NCBI GeneID: 338
• NCBI Accession #: NP_000375.2
• NCBI GenBank Nucleotide #: NM_000384.2
• UniProt Accession #: P04114; O00502; P78479; P78480; P78481; Q13779; Q13785; Q13786; Q13787; Q13788; Q4ZG63; Q53QC8; P78482; Q13789; Q13828; Q59HB3; Q7Z7Q0; Q9UE51; Q9UE52; Q9UE53
• Molecular Weight: 515,605 Da
• NCBI Official Full Name: apolipoprotein B-100
• NCBI Official Synonym Full Names: apolipoprotein B (including Ag(x) antigen)
• NCBI Official Symbol: APOB
• NCBI Official Synonym Symbols: FLDB; LDLCQ4
• NCBI Protein Information: apolipoprotein B-100; apoB-48; apoB-100; apo B-100; mutant Apo B 100; OTTHUMP00000115994; apolipoprotein B48
• UniProt Protein Name: Apolipoprotein B-100
• Protein Family: Apolipoprotein
• UniProt Gene Name: APOB
• UniProt Entry Name: APOB_HUMAN

NCBI Description

This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels. [provided by RefSeq]

Uniprot Description

APOB: Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor. Defects in APOB are a cause of familial hypobetalipoproteinemia type 1 (FHBL1). A disorder characterized by highly reduced plasma concentrations of low density lipoproteins, and dietary fat malabsorption. Clinical presentation may vary from no symptoms to severe gastrointestinal and neurological dysfunction similar to abetalipoproteinemia. Defects in APOB are a cause of familial ligand-defective apolipoprotein B-100 (FDB). FDB is a dominantly inherited disorder of lipoprotein metabolism leading to hypercholesterolemia and increased proneness to coronary artery disease (CAD). The plasma cholesterol levels are dramatically elevated due to impaired clearance of LDL particles by defective APOB/E receptors. Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia.

Protein type: Secreted; Secreted, signal peptide; Carrier

Chromosomal Location of Human Ortholog: 2p24-p23

Cellular Component: Golgi apparatus; extracellular space; endoplasmic reticulum membrane; chylomicron; intracellular membrane-bound organelle; cell soma; endoplasmic reticulum lumen; early endosome; cytoplasm; plasma membrane; extracellular region; endosome membrane; cytosol; actin cytoskeleton

Molecular Function: heparin binding; protein binding; low-density lipoprotein receptor binding; cholesterol transporter activity; phospholipid binding

Biological Process: phototransduction, visible light; lipoprotein catabolic process; response to lipopolysaccharide; response to carbohydrate stimulus; post-embryonic development; response to selenium ion; triacylglycerol catabolic process; retinoid metabolic process; transmembrane transport; cholesterol metabolic process; receptor-mediated endocytosis; nervous system development; cholesterol transport; in utero embryonic development; response to virus; regulation of cholesterol biosynthetic process; lipoprotein metabolic process; cholesterol efflux; cholesterol homeostasis; sperm motility; fertilization; lipoprotein biosynthetic process; lipoprotein transport; artery morphogenesis; spermatogenesis; blood coagulation; triacylglycerol mobilization; leukocyte migration

Disease: Hypercholesterolemia, Autosomal Dominant, Type B; Hypobetalipoproteinemia, Familial, 1