Goat anti-Human Apolipoprotein B, APOB Polyclonal Antibody | anti-APOB antibody

Apolipoprotein B, APOB (including Ag(x) antigen)

Cat. Num. AAA630730

• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by peptide affinity chromatography.
• Synonyms: Apolipoprotein B; APOB; Polyclonal Antibody; APOB (including Ag(x) antigen); Anti -Apolipoprotein B; anti-APOB antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Apolipoprotein B.
• Purity/Purification: Affinity Purified; Purified by peptide affinity chromatography.
• Form/Format: Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

• Applicable Applications for anti-APOB antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Peptide ELISA Titer: 1:32,000; Western Blot: 0.3-1ug/ml
• Immunogen: Synthetic peptide corresponding to C-TDLHLRYQKDKK, from the internal region of human Apolipoprotein B (NP_000375.2).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(MBS630730(0.5µg/ml) staining of Human Serum lysates. Data kindly provided by Yan Xie and NO Davidson, Washington USA. This antibody showed background below 150kDa in serum samples . We call for caution when used for other assays than Western blot.)

Related Product Information for anti-APOB antibody

This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels.

Product Categories/Family for anti-APOB antibody

Antibodies; Abs to Lipoproteins

NCBI and Uniprot Product Information

• NCBI GI #: 113206052
• NCBI GeneID: 396535
• NCBI Accession #: NP_001038098.1
• NCBI GenBank Nucleotide #: NM_001044633.1
• UniProt Accession #: Q197X2; P11682; Q7LZ77
• Molecular Weight: 523,365 Da
• NCBI Official Full Name: apolipoprotein B
• NCBI Official Synonym Full Names: apolipoprotein B (including Ag(x) antigen)
• NCBI Official Symbol: APOB
• NCBI Protein Information: apolipoprotein B
• UniProt Protein Name: Apolipoprotein B
• Protein Family: Apolipoprotein
• UniProt Gene Name: APOB
• UniProt Entry Name: Q197X2_CHICK