Immunohistochemistry (IHC)
((2ug/ml) staining of Mouse (A) and Rat (B) Testes lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.)
anti-Mouse, Rat Cyp2r1 Antibody | anti-CYP2R1 antibody
Goat anti-Cyp2r1 (mouse aa405-418) Antibody
Reactivity
Mouse, Rat
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
Cyp2r1; Goat anti-Cyp2r1 (mouse aa405-418) Antibody; CYP2R1; cytochrome P450; family 2; subfamily R; polypeptide 1; cytochrome P450 2R1; R1; vitamin D 25-hydroxylase; anti-CYP2R1 antibody
Reactivity
Mouse, Rat
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Sequence Length
501
Applicable Applications for anti-CYP2R1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Pep-ELISA: antibody detection limit dilution 1:1000.
WB: Approx 60kDa band observed in Mouse Testis and approx 55-60kDa in Rat Testis lysates. Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature.
WB: Approx 60kDa band observed in Mouse Testis and approx 55-60kDa in Rat Testis lysates. Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature.
Immunogen Sequence
Peptide with sequence C-YSVHFDEKYWKDPD, from the internal region of the protein sequence according to NP_796356.2.
Peptide Sequence + C
C-YSVHFDEKYWKDPD
Epitope
Internal Region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Immunohistochemistry (IHC)
((2ug/ml) staining of Mouse (A) and Rat (B) Testes lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.)
Immunohistochemistry (IHC)
((2ug/ml) staining of Mouse (A) and Rat (B) Testes lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
57.3kDa; 54.2kDa
NCBI Official Full Name
vitamin D 25-hydroxylase
NCBI Official Synonym Full Names
cytochrome P450, family 2, subfamily r, polypeptide 1
NCBI Official Symbol
Cyp2r1
NCBI Protein Information
vitamin D 25-hydroxylase
UniProt Protein Name
Vitamin D 25-hydroxylase
Protein Family
UniProt Gene Name
Cyp2r1
Uniprot Description
Has a D-25-hydroxylase activity on both forms of vitamin D, vitamin D2 and D3.
Research Articles on CYP2R1
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Product Notes
The CYP2R1 cyp2r1 (Catalog #AAA423783) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Cyp2r1 (mouse aa405-418) Antibody reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Cyp2r1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Pep-ELISA: antibody detection limit dilution 1:1000. WB: Approx 60kDa band observed in Mouse Testis and approx 55-60kDa in Rat Testis lysates. Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature. Researchers should empirically determine the suitability of the CYP2R1 cyp2r1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cyp2r1, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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