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Western Blot (WB) (Western Blot: Sample: Rat Muscle Tissue.)

Rabbit anti-Human Apolipoprotein A1 Polyclonal Antibody | anti-APOA1BP antibody

Polyclonal Antibody to Apolipoprotein A1 Binding Protein (APOA1BP)

Gene Names
NAXE; AIBP; PEBEL; YJEFN1; APOA1BP
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Apolipoprotein A1; Polyclonal Antibody; Polyclonal Antibody to Apolipoprotein A1 Binding Protein (APOA1BP); anti-APOA1BP antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against APOA1BP. It has beenselected for its ability to recognize APOA1BP in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- GNN GGDGLVCARH LKLFGYEPTI YYPKRPNKPL FTALVTQCQK MDIPFLGEMP AEPMTIDELY ELVVDAIFGF SFKGDVREPF HSILSVLKGL TVPIASIDIP SGWDVEKGNA GGIQPDLLIS LTAPKKSATQ
Sequence Length
265
Applicable Applications for anti-APOA1BP antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
Fragment
APOA1BP (Gly118~Glu282)
Organism Species
Homo sapiens (Human)
Cross Reactivity
Human, Rat
Conjugate
No Conjugate
Immunogen
Recombinant APOA1BP (Gly118~Glu282) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2072682
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Rat Muscle Tissue.)

Western Blot (WB) (Western Blot: Sample: Rat Muscle Tissue.)

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Glioma Tissue)

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Human Glioma Tissue)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Breast Cancer Tissue.)

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Human Breast Cancer Tissue.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
20,431 Da
NCBI Official Full Name
NAD(P)H-hydrate epimerase
NCBI Official Synonym Full Names
NAD(P)HX epimerase
NCBI Official Symbol
NAXE
NCBI Official Synonym Symbols
AIBP; PEBEL; YJEFN1; APOA1BP
NCBI Protein Information
NAD(P)H-hydrate epimerase
UniProt Protein Name
NAD(P)H-hydrate epimerase
UniProt Gene Name
NAXE
UniProt Synonym Gene Names
YjeF_N1

NCBI Description

The product of this gene interacts with apolipoprotein A-I (apoA-I), the major apolipoprotein of high-density lipoproteins (HDLs). It is secreted into some bodily fluids, and its synthesis and secretion are stimulated in vitro by incubating cells with apoA-I. The human genome contains related pseudogenes. [provided by RefSeq, Jul 2008]

Uniprot Description

APOA1BP: Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. This is a prerequisite for the S- specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX. Belongs to the nnrE/AIBP family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 5.1.99.6; Mitochondrial

Chromosomal Location of Human Ortholog: 1q22

Cellular Component: cilium; cytosol; extracellular region; extracellular space; intracellular membrane-bound organelle; mitochondrial matrix; mitochondrion; nucleoplasm

Molecular Function: metal ion binding; nucleotide binding; protein binding; protein homodimerization activity

Biological Process: NADH metabolic process; NADP metabolic process; nicotinamide nucleotide metabolic process; protein homotetramerization

Disease: Encephalopathy, Progressive, Early-onset, With Brain Edema And/or Leukoencephalopathy

Research Articles on APOA1BP

Similar Products

Product Notes

The APOA1BP naxe (Catalog #AAA2025915) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Apolipoprotein A1 Binding Protein (APOA1BP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Apolipoprotein A1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.5-2ug/ml Immunocytochemistry in formalin fixed cells: 5-20ug/ml Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml Immunohistochemistry in paraffin section: 5-20ug/ml Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the APOA1BP naxe for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- GNN GGDGLVCARH LKLFGYEPTI YYPKRPNKPL FTALVTQCQK MDIPFLGEMP AEPMTIDELY ELVVDAIFGF SFKGDVREPF HSILSVLKGL TVPIASIDIP SGWDVEKGNA GGIQPDLLIS LTAPKKSATQ. It is sometimes possible for the material contained within the vial of "Apolipoprotein A1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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