Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MCM3 antibody at 1:500 dilution.)
Rabbit MCM3 Polyclonal Antibody | anti-MCM3 antibody
MCM3 Antibody
Applications
Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Antigen-Affinity Chromatography
Synonyms
MCM3; Polyclonal Antibody; MCM3 Antibody; Affinity Purified Rabbit Polyclonal MCM3 Antibody; Polyclonal MCM3 antibody; Anti-MCM3 antibody; MGC1157 antibody; MCM-3; MCM 3 antibody; HCC5 antibody; P1-MCM3 antibody; MCM3 minichromosome maintenance deficient 3 antibody; MCM-3 antibody; MCM3 antibody; RLFB antibody; RLF subunit beta antibody; minichromosome maintenance deficient 3 antibody; MCM 3; P1.h antibody; anti-MCM3 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen-Affinity Chromatography
Form/Format
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration
1 mg/ml (varies by lot)
Sequence Length
297
Applicable Applications for anti-MCM3 antibody
Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes
IF: 1:100-1:500
IHC-P: 1:100-1:500
ICC: 1:100-1:500
WB: 1:5000-1:20000
IHC-P: 1:100-1:500
ICC: 1:100-1:500
WB: 1:5000-1:20000
Cross-Reactivity
Human
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MCM3 antibody at 1:500 dilution.)
Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MCM3 antibody at 1:500 dilution.)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: Hep G2) using a 7.5% SDS PAGE gel and MCM3 antibody at a dilution of 1:10000)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: Hep G2) using a 7.5% SDS PAGE gel and MCM3 antibody at a dilution of 1:10000)
Immunohistochemistry (IHC)
(Immunohistochemical staining of paraffin-embedded Cal27 xenograft using MCM3 antibody at a dilution of 1:500)
Immunohistochemistry (IHC)
(Immunohistochemical staining of paraffin-embedded Cal27 xenograft using MCM3 antibody at a dilution of 1:500)
Related Product Information for anti-MCM3 antibody
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression.
Product Categories/Family for anti-MCM3 antibody
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
91 kDa
NCBI Official Full Name
MCM3, partial
Protein Family
Similar Products
Product Notes
The MCM3 (Catalog #AAA5312341) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's MCM3 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB). IF: 1:100-1:500 IHC-P: 1:100-1:500 ICC: 1:100-1:500 WB: 1:5000-1:20000. Researchers should empirically determine the suitability of the MCM3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MCM3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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