Rabbit anti-Human Apolipoprotein A I Polyclonal Antibody | anti-APOA1 antibody

Anti-Human Apolipoprotein A I DyLight 550 conjugated Antibody

Cat. Num. AAA1751270

• Gene Names: APOA1; apo(a)
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: Immunogen Affinity Purified
• Synonyms: Apolipoprotein A I; Polyclonal Antibody; Anti-Human Apolipoprotein A I DyLight 550 conjugated Antibody; Rabbit IgG Polyclonal Anti-Human Apolipoprotein A I Antibody DyLight 550 Conjugated; Flow Validated; Apolipoprotein A-I; Apo-AI; ApoA-I; Apolipoprotein A1; Proapolipoprotein A-I; ProapoA-I; Truncated apolipoprotein A-I; Apolipoprotein A-I (1-242); APOA1; anti-APOA1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: No cross reactivity with other proteins.
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 267

• Applicable Applications for anti-APOA1 antibody: Flow Cytometry (FC/FACS)
• Application Notes: FC/FACS: 1-3ug/1x106 cells
• Immunogen: E Coli-derived human Apolipoprotein A I recombinant protein (Position: D25-Q267). Human Apolipoprotein A I shares 64% and 61.7% amino acid (aa) sequence identity with mouse and rat Apolipoprotein A I, respectively.
• Preparation and Storage: Store at 2-8 degree C for one year. Protect from light. Do not freeze.

Related Product Information for anti-APOA1 antibody

Apolipoprotein A-1, also known as APOA1, is a human protein with a specific role in lipid metabolism. It binds to lipopolysaccharide or endotoxin, and has a major role in the anti-endotoxin function of HDL. The gene is mapped to 11q23. And it is a single polypeptide chain with 243 amino acid residues of known primary amino acid sequence. The ApoA-I protein promotes cholesterol efflux from tissues to the liver for excretion. It is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. ApoA-I is also isolated as a prostacyclin (PGI2) stabilizing factor, and thus may have an anticlotting effect. Defects in the gene encoding it are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Additionally, ApoA-I overexpression promotes macrophage-specific reverse cholesterol transport.

References

1. Brewer, H. B., Jr.; Fairwell, T.; LaRue, A.; Ronan, R.; Houser, A.; Bronzert, T. J.: The amino acid sequence of human apoA-I, an apolipoprotein isolated from high density lipoproteins. Biochem. Biophys. Res. Commun. 80: 623-630, 1978. 2. Ma J, Liao XL, Lou B, Wu MP (2004). "Role of apolipoprotein A-I in protecting against endotoxin toxicity". Acta Biochim. Biophys. Sin. (Shanghai) 36 (6): 419-24. 3. Yui Y, Aoyama T, Morishita H, Takahashi M, Takatsu Y, Kawai C (1988). "Serum prostacyclin stabilizing factor is identical to apolipoprotein A-I (Apo A-I). A novel function of Apo A-I". J. Clin. Invest. 82 (3): 803-7. 4. Zhang, Y.; Zanotti, I.; Reilly, M. P.; Glick, J. M.; Rothblat, G. H.; Rader, D. J.: Overexpression of apolipoprotein A-I promotes reverse transport of cholesterol from macrophages to feces in vivo. Circulation 108: 661-663, 2003.

NCBI and Uniprot Product Information

• NCBI GI #: 4557321
• NCBI GeneID: 335
• NCBI Accession #: NP_000030.1
• NCBI GenBank Nucleotide #: NM_000039.2
• UniProt Accession #: P02647; Q6LDN9; Q6Q785; Q9UCS8; Q9UCT8; A8K866
• Molecular Weight: 30,778 Da
• NCBI Official Full Name: apolipoprotein A-I isoform 1 preproprotein
• NCBI Official Synonym Full Names: apolipoprotein A1
• NCBI Official Symbol: APOA1
• NCBI Official Synonym Symbols: apo(a)
• NCBI Protein Information: apolipoprotein A-I
• UniProt Protein Name: Apolipoprotein A-I
• Protein Family: Apolipoprotein
• UniProt Gene Name: APOA1
• UniProt Synonym Gene Names: Apo-AI; ApoA-I; ProapoA-I

NCBI Description

This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The encoded preproprotein is proteolytically processed to generate the mature protein, which promotes cholesterol efflux from tissues to the liver for excretion, and is a cofactor for lecithin cholesterolacyltransferase (LCAT), an enzyme responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein. [provided by RefSeq, Dec 2015]

Uniprot Description

Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.