Goat APOBEC3G/ARP9 Polyclonal Antibody | anti-APOBEC3G antibody

Goat anti-APOBEC3G/ARP9 Antibody

Cat. Num. AAA423393

• Gene Names: APOBEC3G; A3G; ARCD; ARP9; ARP-9; CEM15; CEM-15; MDS019; bK150C2.7; dJ494G10.1
• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Applications: ELISA, Immunohistochemistry
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: APOBEC3G/ARP9; Polyclonal Antibody; Goat anti-APOBEC3G/ARP9 Antibody; Goat Anti-APOBEC3G Antibody; APOBEC3G; apolipoprotein B mRNA editing enzyme; catalytic polypeptide-like 3G; HGNC: 17357; ARP9; CEM15; FLJ12740; MDS019; OTTHUMP00000028911; bK150C2.7; dJ494G10.1; DNA dC->dU editing enzyme; phorbolin-like protein MDS019; APOBEC3G antibody; catalytic polypeptide-like 3G antibody; HGNC: 17357 antibody; ARP9 antibody; CEM15 antibody; FLJ12740 antibody; MDS019 antibody; OTTHUMP00000028911 antibody; bK150C2.7 antibody; dJ494G10.1 antibody; DNA dC->dU editing enzyme antibody; phorbolin-like protein MDS019 antibody; APOBEC3G / ARP9; anti-APOBEC3G antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: SKWRKLHRDQE
• Sequence Length: 384

• Applicable Applications for anti-APOBEC3G antibody: Peptide ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 64000.; Immunohistochemistry: In paraffin embedded Human Kidney shows weak cytoplasm staining and stronger nuclear staining in a selection of glomerular cells. Also nuclear membrane staining elsewhere. Recommended concentration, 1-2ug/ml.
• Immunogen: Peptide with sequence C-SKWRKLHRDQE, from the internal region of the protein sequence according to NP_068594.1.
• Epitope: Internal region
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

((4ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.)

NCBI and Uniprot Product Information

• NCBI GI #: 13399304
• NCBI GeneID: 60489
• NCBI Accession #: NP_068594.1
• NCBI GenBank Nucleotide #: NM_021822.3
• Molecular Weight: 9,386 Da
• NCBI Official Full Name: DNA dC->dU-editing enzyme APOBEC-3G
• NCBI Official Synonym Full Names: apolipoprotein B mRNA editing enzyme catalytic subunit 3G
• NCBI Official Symbol: APOBEC3G
• NCBI Official Synonym Symbols: A3G; ARCD; ARP9; ARP-9; CEM15; CEM-15; MDS019; bK150C2.7; dJ494G10.1
• NCBI Protein Information: DNA dC->dU-editing enzyme APOBEC-3G
• UniProt Protein Name: DNA dC->dU-editing enzyme APOBEC-3G
• Protein Family: DNA dC->dU-editing enzyme
• UniProt Gene Name: APOBEC3G
• UniProt Synonym Gene Names: APOBEC-related protein; ARCD; ARP-9; CEM15; A3G
• UniProt Entry Name: ABC3G_HUMAN

NCBI Description

This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. It is thought that the proteins may be RNA editing enzymes and have roles in growth or cell cycle control. The protein encoded by this gene has been found to be a specific inhibitor of human immunodeficiency virus-1 (HIV-1) infectivity. [provided by RefSeq, Jul 2008]

Uniprot Description

APOBEC3G: DNA deaminase (cytidine deaminase) that mediates a form of innate resistance to retroviral infections (at least to HIV-1 infection) by triggering G-to-A hypermutation in the newly synthesized viral DNA. The replacements C-to-U in the minus strand DNA of HIV-1 during reverse transcription, leads to G-to-A transitions in the plus strand. The inhibition of viral replication is either due to the degradation of the minus strand before its integration or to the lethality of the hypermutations. Modification of both DNA strands is not excluded. This antiviral activity is neutralized by the virion infectivity factor (VIF), that prevents the incorporation of APOBEC3G into progeny HIV-1 virions by both inhibiting its translation and/or by inducing its ubiquitination and subsequent degradation by the 26S proteasome. May also prevent the transposition of a subset of retroelements. Binds a variety of RNAs, but does not display detectable APOB, NF1 and NAT1 mRNA editing. Belongs to the cytidine and deoxycytidylate deaminase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase; RNA-binding; EC 3.5.4.-; RNA processing

Chromosomal Location of Human Ortholog: 22q13.1-q13.2

Cellular Component: apolipoprotein B mRNA editing enzyme complex; cytoplasm; cytosol; ribonucleoprotein complex

Molecular Function: cytidine deaminase activity; dCTP deaminase activity; deoxycytidine deaminase activity; protein binding; protein homodimerization activity; RNA binding; zinc ion binding

Biological Process: base conversion or substitution editing; cytidine deamination; defense response to virus; innate immune response; negative regulation of retroviral genome replication; negative regulation of viral genome replication; negative regulation of viral reproduction; positive regulation of defense response to virus by host; viral reproduction