Goat anti-Human APOBEC3A Polyclonal Antibody | anti-APOBEC3A antibody

APOBEC3A (Apolipoprotein B mRNA Editing Enzyme Catalytic Polypeptide 3A, APOBEC 3A, APOBEC-3A, ARP3, bK150C2.1, Catalytic Polypeptide-like 3A, Phorbolin 1, PHO1, PHRBN)

Cat. Num. AAA6007825

• Gene Names: APOBEC3A; A3A; ARP3; PHRBN; bK150C2.1
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: APOBEC3A; Polyclonal Antibody; APOBEC3A (Apolipoprotein B mRNA Editing Enzyme Catalytic Polypeptide 3A; APOBEC 3A; APOBEC-3A; ARP3; bK150C2.1; Catalytic Polypeptide-like 3A; Phorbolin 1; PHO1; PHRBN); Anti -APOBEC3A (Apolipoprotein B mRNA Editing Enzyme Catalytic Polypeptide 3A; anti-APOBEC3A antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human APOBEC3A. Species sequence homology: Gorilla (93%), gibbon (86%).
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in Tris saline buffer, pH 7.3, 0.5% BSA, 0.02% sodium azide.

• Applicable Applications for anti-APOBEC3A antibody: ELISA (EL/EIA), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA and Immunohistochemistry.; Dilution: ELISA: 1:8000; Immunohistochemistry (Paraffin): 3.75ug/ml
• Immunogen: Synthetic peptide corresponding to C-TSNFNNGIGRHKTY from human APOBEC3A.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(APOBEC3A IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced retrieval.)

Related Product Information for anti-APOBEC3A antibody

DNA deaminase (cytidine deaminase) with restriction activity against viruses, foreign DNA and mobility of retrotransposons. Exhibits antiviral activity against adeno-associated virus (AAV) and human T-cell leukemia virus type 1 (HTLV-1) and may inhibit the mobility of LTR and non-LTR retrotransposons. Selectively targets single-stranded DNA and can deaminate both methylcytosine and cytosine in foreign DNA. Can induce somatic hypermutation in the nuclear and mitochondrial DNA. May also play a role in the epigenetic regulation of gene expression through the process of active DNA demethylation.

Product Categories/Family for anti-APOBEC3A antibody

Antibodies; Abs to Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 219520199
• NCBI GeneID: 200315
• UniProt Accession #: P31941; Q12807; Q5JZ93; Q9UH18; A0AVM1
• Molecular Weight: 23,012 Da
• NCBI Official Full Name: APOBEC3A protein
• NCBI Official Synonym Full Names: apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3A
• NCBI Official Symbol: APOBEC3A
• NCBI Official Synonym Symbols: A3A; ARP3; PHRBN; bK150C2.1
• NCBI Protein Information: DNA dC->dU-editing enzyme APOBEC-3A; phorbolin 1; phorbolin-1; probable DNA dC->dU-editing enzyme APOBEC-3A
• UniProt Protein Name: DNA dC->dU-editing enzyme APOBEC-3A
• Protein Family: DNA dC->dU-editing enzyme
• UniProt Gene Name: APOBEC3A
• UniProt Synonym Gene Names: A3A
• UniProt Entry Name: ABC3A_HUMAN

NCBI Description

This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. The protein encoded by this gene lacks the zinc binding activity of other family members. The protein plays a role in immunity, by restricting transmission of foreign DNA such as viruses. One mechanism of foreign DNA restriction is deamination of foreign double-stranded DNA cytidines to uridines, which leads to DNA degradation. However, other mechanisms are also thought to be involved, as anti-viral effect is not dependent on deaminase activity. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012]

Uniprot Description

APOBEC3A: a single-stranded DNA cytidine deaminase involved in foreign DNA clearance with restriction activity against viruses, foreign DNA and mobility of retrotransposons. Exhibits antiviral activity against adeno-associated virus (AAV) and human T-cell leukemia virus type 1 (HTLV-1) and may inhibit the mobility of LTR and non-LTR retrotransposons. Selectively targets single-stranded DNA and can deaminate both methylcytosine and cytosine in foreign DNA. Can induce somatic C-to-U hypermutation in nuclear and mitochondrial DNA. May also play a role in the epigenetic regulation of gene expression through the process of active DNA demethylation. Interacts with AGO2. Interacts with TRIB3. Expressed in peripheral leukocytes with higher expression in CD14-positive phagocytic cells. Highly expressed in keratinocytes and in periphery blood monocytes. Also detected in non-lymphoid tissues including lung and adipose tissues. Found at high levels in colorectal adenocarcinoma, Burkitt's lymphoma and chronic myelogenous leukemia. Up-regulated by interferon and CpG single-stranded DNA (at protein level). Belongs to the cytidine and deoxycytidylate deaminase family. 2 isoforms of the human protein are produced by alternative initiation.

Protein type: EC 3.5.4.-; Hydrolase

Chromosomal Location of Human Ortholog: 22q13.1-q13.2

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein binding; zinc ion binding; deoxycytidine deaminase activity; cytidine deaminase activity

Biological Process: negative regulation of viral genome replication; innate immune response; cytidine deamination; defense response to virus