Goat anti-Human APOBEC3A Polyclonal Antibody | anti-APOBEC3A antibody

APOBEC3A (Apolipoprotein B mRNA Editing Enzyme Catalytic Polypeptide 3A, APOBEC 3A, APOBEC-3A, ARP3, BEDP, bK150C2.1, Catalytic Polypeptide-like 3A, Phorbolin 1, PHO1, PHRBN)

Cat. Num. AAA623036

• Gene Names: APOBEC3A; ARP3; PHRBN; bK150C2.1
• Reactivity: Human
• Applications: ELISA
• Purity: Affinity Purified; Purified by affinity chromatography.
• Synonyms: APOBEC3A; Polyclonal Antibody; APOBEC3A (Apolipoprotein B mRNA Editing Enzyme Catalytic Polypeptide 3A; APOBEC 3A; APOBEC-3A; ARP3; BEDP; bK150C2.1; Catalytic Polypeptide-like 3A; Phorbolin 1; PHO1; PHRBN); Anti -APOBEC3A (Apolipoprotein B mRNA Editing Enzyme Catalytic Polypeptide 3A; anti-APOBEC3A antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human Phorbolin 1.
• Purity/Purification: Affinity Purified; Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

• Applicable Applications for anti-APOBEC3A antibody: ELISA (EL/EIA)
• Application Notes: Suitable for use in ELISA.; Dilution: Peptide ELISA: antibody detection limit dilution 1:8,000.; Western Blot: Preliminary experiments gave an approx 28kD band in Human Spleen, Tonsil and Peripheral Blood Mononucleocyte lysates after 0.1ug/ml antibody staining.
• Immunogen: A synthetic peptide from the C-terminus of aa--TSNFNNGIGRHKTY
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Categories/Family for anti-APOBEC3A antibody

Antibodies; Abs to Enzymes; Cytidine Deaminase (APOBEC)

NCBI and Uniprot Product Information

• NCBI GI #: 219520199
• NCBI GeneID: 200315
• UniProt Accession #: P31941; Q12807; Q5JZ93; Q9UH18; A0AVM1
• Molecular Weight: 23,012 Da
• NCBI Official Full Name: APOBEC3A protein
• NCBI Official Synonym Full Names: apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3A
• NCBI Official Symbol: APOBEC3A
• NCBI Official Synonym Symbols: ARP3; PHRBN; bK150C2.1
• NCBI Protein Information: probable DNA dC->dU-editing enzyme APOBEC-3A; phorbolin 1; phorbolin-1; OTTHUMP00000198910
• UniProt Protein Name: Probable DNA dC->dU-editing enzyme APOBEC-3A
• Protein Family: DNA dC->dU-editing enzyme
• UniProt Gene Name: APOBEC3A
• UniProt Entry Name: ABC3A_HUMAN

NCBI Description

This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. The protein encoded by this gene lacks the zinc binding activity of other family members. The protein plays a role in immunity, by restricting transmission of foreign DNA such as viruses. One mechanism of foreign DNA restriction is deamination of foreign double-stranded DNA cytidines to uridines, which leads to DNA degradation. However, other mechanisms are also thought to be involved, as anti-viral effect is not dependent on deaminase activity. One allele of this gene results from the deletion of approximately 29.5 kb of sequence between this gene, APOBEC3A, and the adjacent gene APOBEC3B. The breakpoints of the deletion are within the two genes, so the deletion allele is predicted to have the promoter and coding region of APOBEC3A, but the 3' UTR of APOBEC3B. [provided by RefSeq]

Uniprot Description

APOBEC3A: a single-stranded DNA cytidine deaminase involved in foreign DNA clearance with restriction activity against viruses, foreign DNA and mobility of retrotransposons. Exhibits antiviral activity against adeno-associated virus (AAV) and human T-cell leukemia virus type 1 (HTLV-1) and may inhibit the mobility of LTR and non-LTR retrotransposons. Selectively targets single-stranded DNA and can deaminate both methylcytosine and cytosine in foreign DNA. Can induce somatic C-to-U hypermutation in nuclear and mitochondrial DNA. May also play a role in the epigenetic regulation of gene expression through the process of active DNA demethylation. Interacts with AGO2. Interacts with TRIB3. Expressed in peripheral leukocytes with higher expression in CD14-positive phagocytic cells. Highly expressed in keratinocytes and in periphery blood monocytes. Also detected in non-lymphoid tissues including lung and adipose tissues. Found at high levels in colorectal adenocarcinoma, Burkitt's lymphoma and chronic myelogenous leukemia. Up-regulated by interferon and CpG single-stranded DNA (at protein level). Belongs to the cytidine and deoxycytidylate deaminase family. 2 isoforms of the human protein are produced by alternative initiation.

Protein type: EC 3.5.4.-; Hydrolase

Chromosomal Location of Human Ortholog: 22q13.1-q13.2

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein binding; zinc ion binding; deoxycytidine deaminase activity; cytidine deaminase activity

Biological Process: negative regulation of viral genome replication; innate immune response; cytidine deamination; defense response to virus