Western Blot (WB)
(APOBEC2 Antibody (N-term) western blot analysis in mouse heart tissue lysates (15ug/lane).This demonstrates the APOBEC2 antibody detected APOBEC2 protein (arrow).)
Rabbit anti-Human, Mouse APOBEC2 Polyclonal Antibody | anti-APOBEC2 antibody
APOBEC2, NT (APOBEC2, Probable C->U-editing enzyme APOBEC-2) (FITC)
Gene Names
APOBEC2; ARP1; ARCD1
Reactivity
Human, Mouse
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
APOBEC2; Polyclonal Antibody; NT (APOBEC2; Probable C->U-editing enzyme APOBEC-2) (FITC); Probable C->U-editing enzyme APOBEC-2; anti-APOBEC2 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Applicable Applications for anti-APOBEC2 antibody
Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
FC/FACS: 1:10-50
FLISA: 1:1,000
IHC: 1:50-100
WB: 1:100-500
Applications are based on unconjugated antibody.
FLISA: 1:1,000
IHC: 1:50-100
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
APOBEC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 10-39 amino acids from the N-terminal region of human APOBEC2.
Conjugate
FITC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(APOBEC2 Antibody (N-term) western blot analysis in mouse heart tissue lysates (15ug/lane).This demonstrates the APOBEC2 antibody detected APOBEC2 protein (arrow).)
Western Blot (WB)
(APOBEC2 Antibody (N-term) western blot analysis in mouse heart tissue lysates (15ug/lane).This demonstrates the APOBEC2 antibody detected APOBEC2 protein (arrow).)
Flow Cytometry (FC/FACS)
(APOBEC2 Antibody (N-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(APOBEC2 Antibody (N-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Immunohistochemistry (IHC)
(APOBEC2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the APOBEC2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(APOBEC2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the APOBEC2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-APOBEC2 antibody
APOBEC2 belongs to the cytidine and deoxycytidylate deaminase family. It is probable C to U editing enzyme whose physiological substrate is not yet known. It does not display detectable apoB mRNA editing and has a low intrinsic cytidine deaminase activity.
Product Categories/Family for anti-APOBEC2 antibody
References
Liao, W., et al. Biochem. Biophys. Res. Commun. 260(2):398-404(1999). Oka, K., et al. J. Biol. Chem. 272(3):1456-1460(1997). Lau, P.P., et al. Proc. Natl. Acad. Sci. U.S.A. 91(18):8522-8526(1994).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25,703 Da
NCBI Official Full Name
C->U-editing enzyme APOBEC-2
NCBI Official Synonym Full Names
apolipoprotein B mRNA editing enzyme catalytic subunit 2
NCBI Official Symbol
APOBEC2
NCBI Official Synonym Symbols
ARP1; ARCD1
NCBI Protein Information
C->U-editing enzyme APOBEC-2
UniProt Protein Name
Probable C->U-editing enzyme APOBEC-2
Protein Family
UniProt Gene Name
APOBEC2
UniProt Entry Name
ABEC2_HUMAN
Uniprot Description
APOBEC2: Probable C to U editing enzyme whose physiological substrate is not yet known. Does not display detectable apoB mRNA editing. Has a low intrinsic cytidine deaminase activity. May play a role in the epigenetic regulation of gene expression through the process of active DNA demethylation. Belongs to the cytidine and deoxycytidylate deaminase family.
Protein type: EC 3.5.4.-; Hydrolase; RNA-binding
Chromosomal Location of Human Ortholog: 6p21
Molecular Function: zinc ion binding; RNA binding; cytidine deaminase activity
Biological Process: mRNA modification; cytidine deamination; mRNA processing
Research Articles on APOBEC2
Similar Products
Product Notes
The APOBEC2 apobec2 (Catalog #AAA6274404) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The APOBEC2, NT (APOBEC2, Probable C->U-editing enzyme APOBEC-2) (FITC) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's APOBEC2 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB). FC/FACS: 1:10-50 FLISA: 1:1,000 IHC: 1:50-100 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the APOBEC2 apobec2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "APOBEC2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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