Fucosidase Alpha L1, Tissue (FUCa1) Recombinant Protein | FUCa1 recombinant protein

Recombinant Fucosidase Alpha L1, Tissue (FUCa1)

Cat. Num. AAA2018787

• Gene Names: Fuca1; Afuc; Fuca; 0610006A03Rik; 9530055J05Rik
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Fucosidase Alpha L1; Tissue (FUCa1); Recombinant Fucosidase Alpha L1; FUCa1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- NGFKTQ HFVRAKTMPE LYDLVNSYKP DLIWSDGEWE CPDTYWNSTS FLAWLYNDSP VKDEVIVNDR WGQNCSCHHG GYYNCQDKYK PQSLPDHKWE MCTSMDRASW GYRKDMTMST IAKENEIIEE LVQTVSLGGN YLLNIGPTKD GLIVPIFQER LLAVGKWLQI NGEAIYASKP WRVQSEKNKT VVWYTTKNAT VYATFLYWPE NGIVNLKSPK TTSATKITML GLEGDLSWTQDPLEGVLISL PQLPPTVLPV EFAWTLK
• Sequence Length: 452

• Applicable Applications for FUCa1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Asn185~Lys447 (Accession # Q99LJ1) with two N-terminal Tags, His-tag and T7-tag
• Subcellular Location: Lysosome
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 6.3
• Reconstitution: Reconstitute in sterile ddH2O.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 169808427
• NCBI GeneID: 71665
• NCBI Accession #: NP_077205.3
• NCBI GenBank Nucleotide #: NM_024243.4
• UniProt Accession #: Q99LJ1; Q3UAH8; Q8BN13; Q9DD22; B1AV51
• Molecular Weight: 34.0kDa
• NCBI Official Full Name: tissue alpha-L-fucosidase
• NCBI Official Synonym Full Names: fucosidase, alpha-L- 1, tissue
• NCBI Official Symbol: Fuca1
• NCBI Official Synonym Symbols: Afuc; Fuca; 0610006A03Rik; 9530055J05Rik
• NCBI Protein Information: tissue alpha-L-fucosidase; alpha-L-fucosidase 1; alpha-L-fucosidase I; fucosidase, alpha-L-1, tissue; alpha-L-fucoside fucohydrolase 1
• UniProt Protein Name: Tissue alpha-L-fucosidase
• Protein Family: Tissue alpha-L-fucosidase
• UniProt Gene Name: Fuca1
• UniProt Synonym Gene Names: Fuca; Alpha-L-fucosidase 1
• UniProt Entry Name: FUCO_MOUSE

Uniprot Description

FUCA1: Alpha-L-fucosidase is responsible for hydrolyzing the alpha-1,6-linked fucose joined to the reducing-end N- acetylglucosamine of the carbohydrate moieties of glycoproteins. Defects in FUCA1 are the cause of fucosidosis (FUCA1D). FUCA1D is an autosomal recessive lysosomal storage disease characterized by accumulation of fucose-containing glycolipids and glycoproteins in various tissues. Clinical signs include facial dysmorphism, dysostosis multiplex, moderate hepatomegaly, severe intellectual deficit, deafness, and according to age, angiokeratomas. Belongs to the glycosyl hydrolase 29 family.

Protein type: Glycan Metabolism - other glycan degradation; EC 3.2.1.51; Hydrolase

Cellular Component: lysosome

Molecular Function: fucosidase activity; hydrolase activity; alpha-L-fucosidase activity; hydrolase activity, acting on glycosyl bonds; carbohydrate binding; fucose binding

Biological Process: fucose metabolic process; glycoside catabolic process; metabolic process; carbohydrate metabolic process