Rabbit anti-Human APG4D Polyclonal Antibody | anti-APG4D antibody

APG4D, ID (S341) (ATG4D, APG4D, AUTL4, Cysteine protease ATG4D, AUT-like 4 cysteine endopeptidase, Autophagin-4, Autophagy-related cysteine endopeptidase 4, Autophagy-related protein 4 homolog D) (PE)

Cat. Num. AAA6274257

• Gene Names: ATG4D; APG4D; AUTL4; APG4-D
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: APG4D; Polyclonal Antibody; ID (S341) (ATG4D; AUTL4; Cysteine protease ATG4D; AUT-like 4 cysteine endopeptidase; Autophagin-4; Autophagy-related cysteine endopeptidase 4; Autophagy-related protein 4 homolog D) (PE); ATG4D; Autophagy-related protein 4 homolog D; anti-APG4D antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-APG4D antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: APG4D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 320-346 amino acids from the Central region of human APG4D.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(APG4D Antibody (Center S341) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the APG4D antibody detected the APG4D protein (arrow).)

Related Product Information for anti-APG4D antibody

Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases.

Product Categories/Family for anti-APG4D antibody

Antibodies; Enzymes; Protease (Proteinase; Peptidase)

References

Betin, V.M., et al. J. Cell. Sci. 122 (PT 14), 2554-2566 (2009): Lamesch, P., et al. Genomics 89(3):307-315(2007). Marino, G., et al. J. Biol. Chem. 278(6):3671-3678(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 27903825
• NCBI GeneID: 84971
• NCBI Accession #: NP_116274.3
• NCBI GenBank Nucleotide #: NM_032885.4
• UniProt Accession #: Q86TL0
• Molecular Weight: 52,922 Da
• NCBI Official Full Name: cysteine protease ATG4D
• NCBI Official Synonym Full Names: autophagy related 4D, cysteine peptidase
• NCBI Official Symbol: ATG4D
• NCBI Official Synonym Symbols: APG4D; AUTL4; APG4-D
• NCBI Protein Information: cysteine protease ATG4D; autophagin-4; APG4 autophagy 4 homolog D; AUT-like 4 cysteine endopeptidase; ATG4 autophagy related 4 homolog D; AUT-like 4, cysteine endopeptidase; autophagy-related protein 4 homolog D; cysteine protease involved in autophagy; a
• UniProt Protein Name: Cysteine protease ATG4D
• UniProt Gene Name: ATG4D
• UniProt Synonym Gene Names: APG4D; AUTL4
• UniProt Entry Name: ATG4D_HUMAN

NCBI Description

Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. [provided by RefSeq, Jul 2008]

Uniprot Description

ATG4D: Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Belongs to the peptidase C54 family.

Protein type: EC 3.4.22.-; Ubiquitin conjugating system; Protease; Autophagy

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: mitochondrial matrix; cytoplasm; cytosol

Molecular Function: cysteine-type endopeptidase activity

Biological Process: protein delipidation; apoptosis; mitochondrion degradation; protein processing; C-terminal protein lipidation; protein targeting to membrane; proteolysis; cellular response to nitrogen starvation; autophagic vacuole formation