Western Blot (WB)
(Western blot analysis of Apg10 (Atg10) expression in HeLa cell lysate.)
Rabbit anti-Human Apg10 Polyclonal Antibody | anti-Apg10 antibody
Anti-Apg10 (Atg10) Rabbit pAb
Gene Names
ATG10; APG10; APG10L; pp12616
Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Purity
Affinity Chromatography
Synonyms
Apg10; Polyclonal Antibody; Anti-Apg10 (Atg10) Rabbit pAb; APG10L; ATG10 autophagy related 10 homolog (S. cerevisiae); ATG10 autophagy related 10 homolog; Autophagy related protein 10; anti-Apg10 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Apg10 (Atg10) Antibody detects endogenous levels of total Apg10 (Atg10)
Purity/Purification
Affinity Chromatography
Form/Format
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
220
Applicable Applications for anti-Apg10 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
IP: 1:50
IP: 1:50
Immunogen
A synthesized peptide derived from human Apg10 (Atg10)
Subcellular Location
Cytoplasm
Preparation and Storage
Store at 4 degree C for short term.
Store at-20 degree C for long term.
Avoid freeze/thaw cycle.
Store at-20 degree C for long term.
Avoid freeze/thaw cycle.
Western Blot (WB)
(Western blot analysis of Apg10 (Atg10) expression in HeLa cell lysate.)
Western Blot (WB)
(Western blot analysis of Apg10 (Atg10) expression in HeLa cell lysate.)
Related Product Information for anti-Apg10 antibody
E2-like enzyme involved in autophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for autophagy. Likely serves as an ATG5-recognition molecule. Not involved in ATG12 conjugation to ATG3 (By similarity). Plays a role in adenovirus-mediated cell lysis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25kDa
NCBI Official Full Name
ubiquitin-like-conjugating enzyme ATG10
NCBI Official Synonym Full Names
autophagy related 10
NCBI Official Symbol
ATG10
NCBI Official Synonym Symbols
APG10; APG10L; pp12616
NCBI Protein Information
ubiquitin-like-conjugating enzyme ATG10
UniProt Protein Name
Ubiquitin-like-conjugating enzyme ATG10
UniProt Gene Name
ATG10
UniProt Synonym Gene Names
APG10L; APG10-like
NCBI Description
Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12 (MIM 609608)-ATG5 (MIM 604261) conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A; MIM 601242), a homolog of yeast Apg8, to a membrane-bound form (Nemoto et al., 2003 [PubMed 12890687]).[supplied by OMIM, Mar 2008]
Uniprot Description
E2-like enzyme involved in autophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for autophagy. Likely serves as an ATG5-recognition molecule. Not involved in ATG12 conjugation to ATG3 (). Plays a role in adenovirus-mediated cell lysis.
Research Articles on Apg10
Similar Products
Product Notes
The Apg10 atg10 (Catalog #AAA4750872) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Apg10 (Atg10) Rabbit pAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Apg10 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB: 1:500-1:2000 IP: 1:50. Researchers should empirically determine the suitability of the Apg10 atg10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Apg10, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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