Rabbit APC10 Polyclonal Antibody | anti-ANAPC10 antibody

APC10 Antibody

Cat. Num. AAA151388

• Gene Names: ANAPC10; DOC1; APC10
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: APC10 Antibody is affinity chromatography purified via peptide column.
• Synonyms: APC10; Polyclonal Antibody; APC10 Antibody; DOC1; Anaphase-promoting complex subunit 10; Cyclosome subunit 10; anti-ANAPC10 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: APC10 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid; APC10 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 223

• Applicable Applications for anti-ANAPC10 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: APC10 antibody can be used for detection of APC10 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.; ; Optimal dilutions for each application to be determined by the researcher.
• Conjugate: Unconjugated
• Immunogen: The immunogen is located within amino acids 60-110 of APC10.
• Homology: Predicted species reactivity based on immunogen sequence: Brovine: 100%
• Positive Control: MBS5151649-Mouse Heart Tissue Lysates
• Preparation and Storage: APC10 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(APC10 antibody)

Immunohistochemistry (IHC)

(Immunohistochemistry of APC10 in mouse heart tissue with APC10 antibody at 5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of APC10 in mouse heart tissue with APC10 antibody at 20 μg/mL.)

Related Product Information for anti-ANAPC10 antibody

APC10 Antibody: Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC10 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. It has been suggested that APC10 plays a role to regulate the binding of specific substrates to the APC/C complex, similar to that of coactivators.

NCBI and Uniprot Product Information

• NCBI GI #: 119625457
• NCBI GeneID: 10393
• UniProt Accession #: Q9UM13; Q2V500; Q9UG51; Q9Y5R0; D3DNZ7
• Molecular Weight: 21,252 Da
• NCBI Official Full Name: anaphase promoting complex subunit 10, isoform CRA_b
• NCBI Official Synonym Full Names: anaphase promoting complex subunit 10
• NCBI Official Symbol: ANAPC10
• NCBI Official Synonym Symbols: DOC1; APC10
• NCBI Protein Information: anaphase-promoting complex subunit 10; cyclosome subunit 10
• UniProt Protein Name: Anaphase-promoting complex subunit 10
• UniProt Gene Name: ANAPC10
• UniProt Synonym Gene Names: APC10; APC10
• UniProt Entry Name: APC10_HUMAN

NCBI Description

ANAPC10 is a core subunit of the anaphase-promoting complex (APC), or cyclosome, a ubiquitin protein ligase that is essential for progression through the cell cycle. APC initiates sister chromatid separation by ubiquitinating the anaphase inhibitor securin (PTTG1; MIM 604147) and triggers exit from mitosis by ubiquitinating cyclin B (CCNB1; MIM 123836), the activating subunit of cyclin-dependent kinase-1 (CDK1; MIM 116940) (summary by Wendt et al., 2001 [PubMed 11524682]).[supplied by OMIM, Feb 2011]

Uniprot Description

APC10: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Belongs to the APC10 family.

Protein type: Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 4q31

Cellular Component: nucleoplasm; anaphase-promoting complex; cytosol

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; mitosis; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; cell division; mitotic cell cycle spindle assembly checkpoint; mitotic cell cycle