Rabbit APAF1 Polyclonal Antibody | anti-APAF1 antibody
APAF1 antibody
Gene Names
APAF1; CED4; APAF-1
Applications
Western Blot
Purity
Purified by immunoaffinity purification.
Synonyms
APAF1; Polyclonal Antibody; APAF1 antibody; Rabbit polyclonal APAF 1 antibody; Polyclonal APAF1 antibody; Anti-APAF1 antibody; APAF-1 antibody; Apoptotic protease activating factor 1 antibody; APAF 1; APAF-1; APAF 1 antibody; anti-APAF1 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by immunoaffinity purification.
Form/Format
Supplied in PBS buffer, pH 7.2, containing 30% glycerol,0.05% BSA,0.01% thimerosal.
Concentration
200 ug/ml (varies by lot)
Sequence Length
338
Applicable Applications for anti-APAF1 antibody
Western Blot (WB)
Application Notes
WB: 0.5-4 ug/ml
Immunogen
APAF 1 antibody was raised in Rabbit using synthetic peptide surrounding amino acid 1161 of human APAF-1 as the immunogen.
Cross-Reactivity
Human, Mouse, Rat, Bovine, Rabbit, Hamster, Guinea Pig, Monkey, Horse
Preparation and Storage
Store at-20 degree C. For long-term storage, aliquot and freeze at-70 degree C.
Avoid repeated freeze/thaw cycles.
Avoid repeated freeze/thaw cycles.
Related Product Information for anti-APAF1 antibody
During apoptosis, APAF 1 (apoptosis protease activating factor 1) binds to Cytochrome C (APAF 2), a reaction that requires dATP, and this leads to the formation of a large multimeric complex. The complex recruits pro caspase 9, and activates caspase 9 (APAF 3). Activated caspase 9 in turn cleaves and activates caspase 3, one of the proteases responsible for the proteolytic cleavage of many key proteins in apoptosis. APAF 1 is ubiquitously expressed in human tissues.
Product Categories/Family for anti-APAF1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
37,976 Da
NCBI Official Full Name
apoptotic protease-activating factor 1 isoform e
NCBI Official Synonym Full Names
apoptotic peptidase activating factor 1
NCBI Official Symbol
APAF1
NCBI Official Synonym Symbols
CED4; APAF-1
NCBI Protein Information
apoptotic protease-activating factor 1
UniProt Protein Name
Apoptotic protease-activating factor 1
Protein Family
UniProt Gene Name
APAF1
UniProt Synonym Gene Names
KIAA0413; APAF-1
NCBI Description
This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Uniprot Description
Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.
Research Articles on APAF1
Similar Products
Product Notes
The APAF1 apaf1 (Catalog #AAA5309037) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's APAF1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 0.5-4 ug/ml. Researchers should empirically determine the suitability of the APAF1 apaf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "APAF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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