Rabbit anti-Human AP4B1 Polyclonal Antibody | anti-AP4B1 antibody

AP4B1 Antibody

Cat. Num. AAA7000550

• Gene Names: AP4B1; CPSQ5; SPG47; BETA-4
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified
• Synonyms: AP4B1; Polyclonal Antibody; AP4B1 Antibody; AP-4 complex subunit beta-1; AP-4 adaptor complex subunit beta; Adaptor-related protein complex 4 subunit beta-1; Beta subunit of AP-4; Beta4-adaptin; anti-AP4B1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purified
• Form/Format: Liquid
• Sequence Length: 739

• Applicable Applications for anti-AP4B1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Recommended dilution: WB:1:500-2000, IHC:1:20-1:200
• Immunogen: Recombinant human AP-4 complex subunit beta-1 protein
• Conjugation: Non-conjugated
• Storage Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-18460
• Preparation and Storage: Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.

Western Blot (WB)

(Western blotAll lanes: AP4B1 antibody at 3ug/mlLane 1: HepG-2 whole cell lysateLane 2: U251 whole cell lysateLane 3: LO2 whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 84,35 kDaObserved band size: 83 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human testis tissue using MBS7000550 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human adrenal gland tissue using MBS7000550 at dilution of 1:100)

Related Product Information for anti-AP4B1 antibody

Subunit of novel type of clathrin- or non-clathrin-associated protein coat involved in targeting proteins from the trans-Golgi network (TGN) to the endosomal-lysosomal system.

NCBI and Uniprot Product Information

• NCBI GI #: 359806940
• NCBI GeneID: 10717
• NCBI Accession #: NP_001240781.1
• NCBI GenBank Nucleotide #: NM_001253852.2
• UniProt Accession #: Q9Y6B7; Q59EJ4; Q96CL6; B7Z4X3
• Molecular Weight: 34,403 Da
• NCBI Official Full Name: AP-4 complex subunit beta-1 isoform 1
• NCBI Official Synonym Full Names: adaptor related protein complex 4 beta 1 subunit
• NCBI Official Symbol: AP4B1
• NCBI Official Synonym Symbols: CPSQ5; SPG47; BETA-4
• NCBI Protein Information: AP-4 complex subunit beta-1
• UniProt Protein Name: AP-4 complex subunit beta-1
• Protein Family: AP-4 complex
• UniProt Gene Name: AP4B1
• UniProt Entry Name: AP4B1_HUMAN

NCBI Description

This gene encodes a subunit of a heterotetrameric adapter-like complex 4 that is involved in targeting proteins from the trans-Golgi network to the endosomal-lysosomal system. Mutations in this gene are associated with cerebral palsy spastic quadriplegic type 5 (CPSQ5) disorder. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

AP4B1: Subunit of novel type of clathrin- or non-clathrin- associated protein coat involved in targeting proteins from the trans-Golgi network (TGN) to the endosomal-lysosomal system. Defects in AP4B1 are the cause of cerebral palsy spastic quadriplegic type 5 (CPSQ5). CPSQ5 is a neurodevelopmental disorder characterized by neonatal hypotonia that progresses to hypertonia and spasticity, and severe mental retardation with poor or absent speech development. Belongs to the adaptor complexes large subunit family.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 1p13.2

Cellular Component: clathrin adaptor complex; coated pit; trans-Golgi network; trans-Golgi network membrane

Molecular Function: protein binding; protein transporter activity; transporter activity

Biological Process: intracellular protein transport; post-Golgi vesicle-mediated transport

Disease: Spastic Paraplegia 47, Autosomal Recessive