Western Blot
Synaptopodin Monoclonal Antibody
MAb to Synaptopodin
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Protein A chromatography
Synonyms
Synaptopodin; Monoclonal Antibody; MAb to Synaptopodin; Monoclonal Antibody to Synaptopodin; anti-Synaptopodin antibody
Host
Host: Mouse
Source: Cell Culture
Source: Cell Culture
Clonality
Monoclonal
Isotype
IgG1
Clone Number
G1D4
Specificity
Recognizes differentiated podocytes (glomerular visceral epithelial cells) in vivo and in vitro (weaker additional reaction with arterial endothelial cells), co-localization with alpha-actinin. Does not react with parietal cells. Reacts with a subset of exclusively telencephalic synapses. Differentiation-dependent expression during postnatal maturation of rat brain. Differentiation-dependent expression in cultured hippocampal neurons. Reacts specifically with synaptopodin, a prolin-rich actin-binding protein with 2 binding sites for actin. Cross-reacts with human, bovine, rat, mouse, guinea pig and gerbil. Does not cross-react with rabbit, frog or chicken
Purity/Purification
Protein A chromatography
Form/Format
Purified, Lyophilized. Reconstitute with 1ml distilled water.
Concentration
50ug/ml (prior to lyophilization) (varies by lot)
Applicable Applications for anti-Synaptopodin antibody
Immunofluorescence, Immunohistochemistry, Western Blot
Application Notes
Suitable for use in immunofluorescence, immunohistochemistry (1:5 - 1:10 with frozen sections or paraffin-embedded tissue after microwave treatment), and Western blot
Immunogen
Isolated rat kidney glomeruli
Affinity Constant
None
Buffer
Lyophilized from PBS, pH 7.4 containing 0.5% BSA
Preservative
0.09% Sodium azide
Warning
This product contains sodium azide, which has been classified as Xn (Harmful), in European Directive 67/548/EEC in the concentration range of 0.1-1.0%. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains.
Preparation and Storage
Store lyophilized product at 2-8 degree C. After reconstitution, aliquot and store at -20 degree C. Avoid multiple freeze/thaw cycles.
Western Blot
Western Blot
References
1. Mundel, P., et al., (1991), "Podocytes in Glomerulus of Rat Kidney Express a Characteristic 44 kD Protein", J. Histochem. and Cytochem., Vol. 39, No. 8, 1047-1056.
2. Mundel, P., et al., (1995), "Structure and function of podocytes: an update", Anat. Embryol., 192, 385-397.
3. Mundel, P., et al., (1997), "Phenotypic conversion and differention of human and rat podocytes in vitro", J. Am. Soc. Nephrol., 8, 89708-89705.
4. Mundel, P., et al., (1997), "Synaptopodin, an actin-associated protein in telencephalic dendrites and in renal podocytes", J. Cell Biol., Vol 139(1), 193-204.
5. Mundel, P., et al., (1997), "Rearrangements of cytoskeleton and cell contacts induce process formation and postmitotic differentiation of conditionally immortalized mouse podocyte cell lines", Exp. Cell Res., 236, 248-258.
6. Kobayashi, N., et al., (1998), "Non-uniform microtubular polarity, established by CHO1/MKLP1 motor protein, is necessary for process formation of podocytes", J. Cell Biol., 143, 1961-1970.
7. Barisoni, L., et al., (1999), "The dysregulated podocyte phenotype: a novel concept in the pathogenesis of collapsing idiopathic focal segmental glomerulosclerosis and HIV-associated nephropathy", J. Am. Soc. Nephrol., 10, 51-61.
8. Kihara, I., et al., "Origin of hyperplastic epithelial cells in idiopathic collapsing glomerulopathy", Histopathology, 67(6), 537-547.
Asanuma, K., et al., (2005), "Synaptopodin regulates the actin-binding activity of alpha-actinin in an isoform-specific manner", J. Clin. Invest., 115, 1188-1198
2. Mundel, P., et al., (1995), "Structure and function of podocytes: an update", Anat. Embryol., 192, 385-397.
3. Mundel, P., et al., (1997), "Phenotypic conversion and differention of human and rat podocytes in vitro", J. Am. Soc. Nephrol., 8, 89708-89705.
4. Mundel, P., et al., (1997), "Synaptopodin, an actin-associated protein in telencephalic dendrites and in renal podocytes", J. Cell Biol., Vol 139(1), 193-204.
5. Mundel, P., et al., (1997), "Rearrangements of cytoskeleton and cell contacts induce process formation and postmitotic differentiation of conditionally immortalized mouse podocyte cell lines", Exp. Cell Res., 236, 248-258.
6. Kobayashi, N., et al., (1998), "Non-uniform microtubular polarity, established by CHO1/MKLP1 motor protein, is necessary for process formation of podocytes", J. Cell Biol., 143, 1961-1970.
7. Barisoni, L., et al., (1999), "The dysregulated podocyte phenotype: a novel concept in the pathogenesis of collapsing idiopathic focal segmental glomerulosclerosis and HIV-associated nephropathy", J. Am. Soc. Nephrol., 10, 51-61.
8. Kihara, I., et al., "Origin of hyperplastic epithelial cells in idiopathic collapsing glomerulopathy", Histopathology, 67(6), 537-547.
Asanuma, K., et al., (2005), "Synaptopodin regulates the actin-binding activity of alpha-actinin in an isoform-specific manner", J. Clin. Invest., 115, 1188-1198
NCBI and Uniprot Product Information
Uniprot Description
SYNPO: an actin-associated protein that may play a role in modulating actin-based shape and motility of dendritic spines and renal podocyte foot processes. Seems to be essential for the formation of spine apparatuses in spines of telencephalic neurons, which is involved in synaptic plasticity.
Protein type: Actin-binding; Cytoskeletal
Chromosomal Location of Human Ortholog: 5q33.1
Cellular Component: postsynaptic membrane; tight junction; cytoplasm; postsynaptic density; stress fiber; dendritic spine; perikaryon; actin cytoskeleton
Molecular Function: protein binding; actin binding
Biological Process: regulation of stress fiber formation; positive regulation of actin filament bundle formation
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Product Notes
The Synaptopodin (Catalog #AAA313029) is an Antibody produced from Host: Mouse Source: Cell Culture and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Synaptopodin can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence, Immunohistochemistry, Western Blot. Suitable for use in immunofluorescence, immunohistochemistry (1:5 - 1:10 with frozen sections or paraffin-embedded tissue after microwave treatment), and Western blot. Researchers should empirically determine the suitability of the Synaptopodin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Synaptopodin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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