Rabbit AP2M1 Polyclonal Antibody | anti-AP2M1 antibody

AP2M1 (Adaptor-related Protein Complex 2 mu 1, AP50, CLAPM1, Mu2) (HRP)

Cat. Num. AAA6406667

• Gene Names: AP2M1; mu2; AP50; CLAPM1
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: AP2M1; Polyclonal Antibody; AP2M1 (Adaptor-related Protein Complex 2 mu 1; AP50; CLAPM1; Mu2) (HRP); anti-AP2M1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes AP2M1. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-AP2M1 antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IHC: 1:50-1:200; WB: 1:500-1:2000; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human AP2M1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of HepG2 cell line, using AP2M1 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical of paraffin-embedded rat kidney using AP2M1 antibody at dilution of 1:100 (200x lens).)

Product Categories/Family for anti-AP2M1 antibody

Antibodies; Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 51316978
• NCBI GeneID: 1173
• UniProt Accession #: Q96CW1; P20172; P53679; A6NE12; D3DNT1
• Molecular Weight: 435
• NCBI Official Full Name: AP-2 complex subunit mu
• NCBI Official Synonym Full Names: adaptor-related protein complex 2, mu 1 subunit
• NCBI Official Symbol: AP2M1
• NCBI Official Synonym Symbols: mu2; AP50; CLAPM1
• NCBI Protein Information: AP-2 complex subunit mu; adaptin-mu2; AP-2 mu chain; AP-2 mu 2 chain; HA2 50 kDA subunit; clathrin coat adaptor protein AP50; clathrin coat assembly protein AP50; clathrin coat-associated protein AP50; adaptor protein complex AP-2 subunit mu; clathrin ada
• UniProt Protein Name: AP-2 complex subunit mu
• UniProt Gene Name: AP2M1
• UniProt Synonym Gene Names: CLAPM1; KIAA0109
• UniProt Entry Name: AP2M1_HUMAN

NCBI Description

This gene encodes a subunit of the heterotetrameric coat assembly protein complex 2 (AP2), which belongs to the adaptor complexes medium subunits family. The encoded protein is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. The encoded protein may also play an important role in regulating the intracellular trafficking and function of CTLA-4 protein. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

AP2M1: a protein of the adaptor complexes medium subunit family. A subunit of the heterotetrameric coat assembly protein complex 2 (AP2) which links clathrin to receptors in coated vesicles. Interacts with the cytoplasmic tails of membrane proteins and polyphosphoinositide-containing lipids. Is not required for clathrin-coated vesicle formation at the plasma membrane, but that it is one of several endocytic adaptors required for the uptake of certain cargo proteins. Is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. AP-2 is a heterotetramer composed of two large chains (alpha and beta), a medium chain (AP50) and a small chain (AP17).

Protein type: Vesicle; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 3q28

Cellular Component: mitochondrion; lysosomal membrane; plasma membrane; AP-2 adaptor complex; cytosol

Molecular Function: protein binding; low-density lipoprotein receptor binding; transporter activity; signal sequence binding; lipid binding

Biological Process: epidermal growth factor receptor signaling pathway; negative regulation of epidermal growth factor receptor signaling pathway; intracellular protein transport; axon guidance; synaptic transmission; viral reproduction; nerve growth factor receptor signaling pathway; ephrin receptor signaling pathway; antigen processing and presentation of exogenous peptide antigen via MHC class II; regulation of defense response to virus by virus