Western Blot (WB)
(All lanes : Anti-ANXA2 Antibody (N-term) at 1:2000 dilutionLane 1: Hela whole cell lysatesLane 2: MCF-7 whole cell lysatesLane 3: NIH/3T3 whole cell lysatesLane 4: SH-SY5Y whole cell lysatesLane 5: SK-BR-3 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 39 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit ANXA2 Polyclonal Antibody | anti-ANXA2 antibody
ANXA2 Antibody (N-term)
Western Blot (WB)
(All lanes : Anti-ANXA2 Antibody (N-term) at 1:2000 dilutionLane 1: Hela whole cell lysatesLane 2: MCF-7 whole cell lysatesLane 3: NIH/3T3 whole cell lysatesLane 4: SH-SY5Y whole cell lysatesLane 5: SK-BR-3 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 39 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-ANXA2 Antibody (N-term) at 1:2000 dilutionLane 1: Hela whole cell lysatesLane 2: MCF-7 whole cell lysatesLane 3: NIH/3T3 whole cell lysatesLane 4: SH-SY5Y whole cell lysatesLane 5: SK-BR-3 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 39 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Western blot analysis of ANXA2 (arrow) using rabbit polyclonal ANXA2 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ANXA2 gene.)
Western Blot (WB)
(Western blot analysis of ANXA2 (arrow) using rabbit polyclonal ANXA2 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ANXA2 gene.)
Western Blot (WB)
(Western blot analysis of anti-ANXA2 Antibody (N-term) in mouse lung tissue lysates (35ug/lane). ANXA2(arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of anti-ANXA2 Antibody (N-term) in mouse lung tissue lysates (35ug/lane). ANXA2(arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ANXA2 antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ANXA2 antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Western Blot (WB)
(ANXA2 Antibody (N-term) western blot analysis in 293,MDA-MB231 cell line lysates (35ug/lane).This demonstrates the ANXA2 antibody detected the ANXA2 protein (arrow).)
Western Blot (WB)
(ANXA2 Antibody (N-term) western blot analysis in 293,MDA-MB231 cell line lysates (35ug/lane).This demonstrates the ANXA2 antibody detected the ANXA2 protein (arrow).)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. This gene has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
ANXA2: a calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. Heterotetramer containing 2 light chains of S100A10 2 heavy chains of ANXA2. May cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis. Annexins are a family of structurally related proteins whose common property is calcium-dependent binding to phospholipids. There are at least ten different annexins in mammalian species. Annexins do not contain signal peptides, yet some annexins (A1, A2 and A5) appear to be secreted in a physiologically regulated fashion.
Protein type: Calcium-binding; Motility/polarity/chemotaxis; Lipid-binding
Chromosomal Location of Human Ortholog: 15q22.2
Cellular Component: extrinsic to plasma membrane; extracellular space; cell surface; protein complex; lysosomal membrane; late endosome membrane; early endosome; lipid particle; cell cortex; lipid raft; extracellular matrix; ruffle; membrane; perinuclear region of cytoplasm; plasma membrane; melanosome; basement membrane; midbody; nucleus; endosome; sarcolemma; vesicle
Molecular Function: protein binding; phosphatidylinositol-4,5-bisphosphate binding; calcium-dependent phospholipid binding; phospholipase A2 inhibitor activity; cytoskeletal protein binding; calcium ion binding; calcium-dependent protein binding; Rab GTPase binding
Biological Process: positive regulation of fibroblast proliferation; fibrinolysis; collagen fibril organization; negative regulation of catalytic activity; positive regulation of vesicle fusion; protein heterotetramerization; angiogenesis; positive regulation of binding; positive regulation of protein amino acid phosphorylation; lipid raft formation; body fluid secretion; membrane budding
Research Articles on ANXA2
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Product Notes
The ANXA2 anxa2 (Catalog #AAA9205365) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 19-49. The ANXA2 Antibody (N-term) reacts with Human, mouse (Predicted Reactivity: Bovine, Pig, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's ANXA2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the ANXA2 anxa2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ANXA2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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