Human Rho GDP Dissociation Inhibitor Beta (ARHGDIb) ELISA Kit | ARHGDIb elisa kit

Human Rho GDP Dissociation Inhibitor Beta (ARHGDIb) ELISA Kit

Cat. Num. AAA2021844

• Gene Names: ARHGDIB; D4; GDIA2; GDID4; LYGDI; Ly-GDI; RAP1GN1; RhoGDI2
• Reactivity: Human
• Synonyms: Rho GDP Dissociation Inhibitor Beta (ARHGDIb); Human Rho GDP Dissociation Inhibitor Beta (ARHGDIb) ELISA Kit; D4; GDIA2; GDID4; LYGDI; Ly-GDI; RAP1GN1; RhoGDI2; ARHGDIb elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Rho GDP Dissociation Inhibitor Beta (ARHGDIb).; No significant cross-reactivity or interference between Rho GDP Dissociation Inhibitor Beta (ARHGDIb) and analogues was observed.
• Sequence Length: 201

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.625-40ng/mL
• Sensitivity: < 0.241ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Rho GDP Dissociation Inhibitor Beta (ARHGDIb) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Rho GDP Dissociation Inhibitor Beta (ARHGDIb) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ARHGDIb elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rho GDP Dissociation Inhibitor Beta (ARHGDIb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Rho GDP Dissociation Inhibitor Beta (ARHGDIb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rho GDP Dissociation Inhibitor Beta (ARHGDIb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rho GDP Dissociation Inhibitor Beta (ARHGDIb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for ARHGDIb elisa kit

Tumor immunity

NCBI and Uniprot Product Information

• NCBI GI #: 20379030
• NCBI GeneID: 397
• UniProt Accession #: P52566; B5BU79
• Molecular Weight: 22,988 Da
• NCBI Official Full Name: Rho GDP dissociation inhibitor beta
• NCBI Official Synonym Full Names: Rho GDP dissociation inhibitor (GDI) beta
• NCBI Official Symbol: ARHGDIB
• NCBI Official Synonym Symbols: D4; GDIA2; GDID4; LYGDI; Ly-GDI; RAP1GN1; RhoGDI2
• NCBI Protein Information: rho GDP-dissociation inhibitor 2
• UniProt Protein Name: Rho GDP-dissociation inhibitor 2
• Protein Family: Rho GDP-dissociation inhibitor
• UniProt Gene Name: ARHGDIB
• UniProt Synonym Gene Names: GDIA2; GDID4; RAP1GN1; Rho GDI 2
• UniProt Entry Name: GDIR2_HUMAN

NCBI Description

Members of the Rho (or ARH) protein family (see MIM 165390) and other Ras-related small GTP-binding proteins (see MIM 179520) are involved in diverse cellular events, including cell signaling, proliferation, cytoskeletal organization, and secretion. The GTP-binding proteins are active only in the GTP-bound state. At least 3 classes of proteins tightly regulate cycling between the GTP-bound and GDP-bound states: GTPase-activating proteins (GAPs), guanine nucleotide-releasing factors (GRFs), and GDP-dissociation inhibitors (GDIs). The GDIs, including ARHGDIB, decrease the rate of GDP dissociation from Ras-like GTPases (summary by Scherle et al., 1993 [PubMed 8356058]).[supplied by OMIM, Dec 2010]

Uniprot Description

RhoGDI beta: regulates the GDP/GTP exchange reaction of Rho proteins by inhibiting the dissociation of GDP from them, keeping these small GTPases in an inactive state. The GDIs are made up of two domains: a flexible N-terminal domain of about 70 amino acid residues and a folded 134-residue C-terminal domain. Regulates the GTP/GDP cycling of glucose-responsive small GTPases. Its phosphorylation on Y156, S101, and S174 mediates the cycling of Cdc42 and Rac1 to regulate second-phase insulin secretion. Interacts with WNK1.

Protein type: Motility/polarity/chemotaxis; Cell adhesion; G protein regulator, misc.

Chromosomal Location of Human Ortholog: 12p12.3

Cellular Component: cytoskeleton; cytoplasm; cytoplasmic membrane-bound vesicle; cytosol

Molecular Function: Rho GDP-dissociation inhibitor activity; GTPase activator activity

Biological Process: regulation of small GTPase mediated signal transduction; regulation of catalytic activity; small GTPase mediated signal transduction; multicellular organismal development; immune response; negative regulation of cell adhesion; actin cytoskeleton organization and biogenesis; cell motility; Rho protein signal transduction; positive regulation of GTPase activity