Rabbit anti-Human ANXA2 Polyclonal Antibody | anti-ANXA2 antibody

ANXA2, CT (Annexin A2, Annexin-2, Annexin II, Lipocortin II, Calpactin I Heavy Chain, Chromobindin-8, p36, Protein I, Placental Anticoagulant Protein IV, PAP-IV, ANX2, ANX2L4, CAL1H, LPC2D) (MaxLight 550)

Cat. Num. AAA6463248

• Gene Names: ANXA2; P36; ANX2; LIP2; LPC2; CAL1H; LPC2D; ANX2L4; PAP-IV; HEL-S-270
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: ANXA2; Polyclonal Antibody; CT (Annexin A2; Annexin-2; Annexin II; Lipocortin II; Calpactin I Heavy Chain; Chromobindin-8; p36; Protein I; Placental Anticoagulant Protein IV; PAP-IV; ANX2; ANX2L4; CAL1H; LPC2D) (MaxLight 550); anti-ANXA2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ANXA2.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.

• Applicable Applications for anti-ANXA2 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-1:50; FLISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human ANXA2 (KLH).
• Conjugate: MaxLight550
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-ANXA2 Antibody (C-term) in A2058 cell line lysates (35ug/lane). ANXA2(arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ANXA2 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Western Blot (WB)

(Western blot analysis of ANXA2 (arrow) using rabbit polyclonal ANXA2 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ANXA2 gene.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of ANXA2 Antibody (C-term) with A2058 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Product Categories/Family for anti-ANXA2 antibody

Antibodies; Annexin

References

1. He, K.L., J. Biol. Chem. 283(28), 19192-19200 (2008). 2. Tamma, G., Pflugers Arch. 456(4), 729-736 (2008). 3. Gou, D., J. Biol. Chem. 283(19), 13156-13164 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 4757756
• NCBI GeneID: 302
• NCBI Accession #: NP_004030
• NCBI GenBank Nucleotide #: NM_004039
• UniProt Accession #: P07355
• Molecular Weight: 37kDa
• NCBI Official Full Name: annexin A2 isoform 2
• NCBI Official Synonym Full Names: annexin A2
• NCBI Official Symbol: ANXA2
• NCBI Official Synonym Symbols: P36; ANX2; LIP2; LPC2; CAL1H; LPC2D; ANX2L4; PAP-IV; HEL-S-270
• NCBI Protein Information: annexin A2
• UniProt Protein Name: Annexin A2
• Protein Family: Annexin
• UniProt Gene Name: ANXA2
• UniProt Synonym Gene Names: ANX2; ANX2L4; CAL1H; LPC2D; PAP-IV
• UniProt Entry Name: ANXA2_HUMAN

NCBI Description

This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. This gene has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ANXA2: a calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. Heterotetramer containing 2 light chains of S100A10 2 heavy chains of ANXA2. May cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis. Annexins are a family of structurally related proteins whose common property is calcium-dependent binding to phospholipids. There are at least ten different annexins in mammalian species. Annexins do not contain signal peptides, yet some annexins (A1, A2 and A5) appear to be secreted in a physiologically regulated fashion.

Protein type: Motility/polarity/chemotaxis; Lipid-binding; Calcium-binding

Chromosomal Location of Human Ortholog: 15q22.2

Cellular Component: extrinsic to plasma membrane; extracellular space; protein complex; cell surface; lysosomal membrane; late endosome membrane; early endosome; lipid particle; cell cortex; lipid raft; extracellular matrix; ruffle; membrane; perinuclear region of cytoplasm; melanosome; plasma membrane; basement membrane; midbody; nucleus; sarcolemma; endosome; vesicle

Molecular Function: phosphatidylinositol-4,5-bisphosphate binding; protein binding; calcium-dependent phospholipid binding; phospholipase A2 inhibitor activity; cytoskeletal protein binding; calcium ion binding; calcium-dependent protein binding; Rab GTPase binding

Biological Process: positive regulation of fibroblast proliferation; fibrinolysis; collagen fibril organization; negative regulation of catalytic activity; protein heterotetramerization; positive regulation of vesicle fusion; angiogenesis; positive regulation of binding; positive regulation of protein amino acid phosphorylation; lipid raft formation; body fluid secretion; membrane budding