Rabbit anti-Human ANTXR1 Polyclonal Antibody | anti-ANTXR1 antibody

ANTXR1 (Y382) (Anthrax Toxin Receptor 1, Tumor Endothelial Marker 8, ATR, TEM8) (PE)

Cat. Num. AAA6463350

• Gene Names: ANTXR1; ATR; GAPO; TEM8
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: ANTXR1; Polyclonal Antibody; ANTXR1 (Y382) (Anthrax Toxin Receptor 1; Tumor Endothelial Marker 8; ATR; TEM8) (PE); anti-ANTXR1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ANTXR1.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-ANTXR1 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-1:50; FLISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to amino acid residues surrounding Y382 of human ANTXR1 (KLH).
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-ANTXR1 Antibody (Y382) in Hela cell line lysates (35ug/lane). ANTXR1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ANTXR1 Antibody (Y382), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-ANTXR1 antibody

Antibodies; Infectious Disease Anthrax

References

1. Werner, E., J. Biol. Chem. 281(32), 23227-23236 (2006). 2. Wei, W., Cell 124(6), 1141-1154 (2006). 3. Rainey, G.J., Proc. Natl. Acad. Sci. USA 102(37), 13278-13283 (2005).

NCBI and Uniprot Product Information

• NCBI GI #: 14149904
• NCBI GeneID: 84168
• NCBI Accession #: NP_115584
• NCBI GenBank Nucleotide #: NM_032208
• UniProt Accession #: Q9H6X2
• Molecular Weight: 60kDa
• NCBI Official Full Name: anthrax toxin receptor 1 isoform 1
• NCBI Official Synonym Full Names: ANTXR cell adhesion molecule 1
• NCBI Official Symbol: ANTXR1
• NCBI Official Synonym Symbols: ATR; GAPO; TEM8
• NCBI Protein Information: anthrax toxin receptor 1
• UniProt Protein Name: Anthrax toxin receptor 1
• Protein Family: Anthrax toxin receptor
• UniProt Gene Name: ANTXR1
• UniProt Synonym Gene Names: ATR; TEM8
• UniProt Entry Name: ANTR1_HUMAN

NCBI Description

This gene encodes a type I transmembrane protein and is a tumor-specific endothelial marker that has been implicated in colorectal cancer. The encoded protein has been shown to also be a docking protein or receptor for Bacillus anthracis toxin, the causative agent of the disease, anthrax. The binding of the protective antigen (PA) component, of the tripartite anthrax toxin, to this receptor protein mediates delivery of toxin components to the cytosol of cells. Once inside the cell, the other two components of anthrax toxin, edema factor (EF) and lethal factor (LF) disrupt normal cellular processes. Three alternatively spliced variants that encode different protein isoforms have been described. [provided by RefSeq, Oct 2008]