Mouse Lin-28 Homolog A (LIN28A) RTU ELISA Kit | LIN28A rtu elisa kit

Mouse Lin-28 Homolog A (LIN28A) ELISA Kit

Cat. Num. AAA4500774

• Gene Names: Lin28a; Lin28; Tex17; Lin-28; Gm10299; lin-28A; AL024421
• Reactivity: Mouse
• Synonyms: Lin-28 Homolog A (LIN28A); Mouse Lin-28 Homolog A (LIN28A) ELISA Kit; ZCCHC1; CSDD1; LIN28; Zinc finger CCHC domain-containing protein 1; LIN28A rtu elisa kit

• Reactivity: Mouse
• Sequence Length: 209

• Assay Type: Sandwich
• Samples: Tissue Homogenates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.049ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LIN28A rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to LIN28A. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to LIN28A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain LIN28A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of LIN28A in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 22003878
• NCBI GeneID: 83557
• NCBI Accession #: NP_665832.1
• NCBI GenBank Nucleotide #: NM_145833.1
• UniProt Accession #: Q8K3Y3; Q6NV62
• Molecular Weight: 22,720 Da
• NCBI Official Full Name: protein lin-28 homolog A
• NCBI Official Synonym Full Names: lin-28 homolog A (C. elegans)
• NCBI Official Symbol: Lin28a
• NCBI Official Synonym Symbols: Lin28; Tex17; Lin-28; Gm10299; lin-28A; AL024421
• NCBI Protein Information: protein lin-28 homolog A
• UniProt Protein Name: Protein lin-28 homolog A
• Protein Family: Protein
• UniProt Gene Name: Lin28a
• UniProt Synonym Gene Names: Lin28; Tex17; Lin-28A

Uniprot Description

RNA-binding protein that inhibits processing of pre-let-7 miRNAs and regulates translation of mRNAs that control developmental timing, pluripotency and metabolism (PubMed:17473174, PubMed:18604195, PubMed:18566191, PubMed:18292307, PubMed:19703396, PubMed:23102813, PubMed:24209617). Seems to recognize a common structural G-quartet (G4) feature in its miRNA and mRNA targets (PubMed:26045559). 'Translational enhancer' that drives specific mRNAs to polysomes and increases the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in mRNA stabilization. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression (PubMed:17473174). Suppressor of microRNA (miRNA) biogenesis, including that of let-7, miR107, miR-143 and miR-200c. Specifically binds the miRNA precursors (pre-miRNAs), recognizing an 5'-GGAG-3' motif found in pre-miRNA terminal loop, and recruits ZCCHC11/TUT4 uridylyltransferase. This results in the terminal uridylation of target pre-miRNAs. Uridylated pre-miRNAs fail to be processed by Dicer and undergo degradation. The repression of let-7 expression is required for normal development and contributes to maintain the pluripotent state by preventing let-7-mediated differentiation of embryonic stem cells (PubMed:19703396). Localized to the periendoplasmic reticulum area, binds to a large number of spliced mRNAs and inhibits the translation of mRNAs destined for the ER, reducing the synthesis of transmembrane proteins, ER or Golgi lumen proteins, and secretory proteins (PubMed:23102813). Binds to and enhances the translation of mRNAs for several metabolic enzymes, such as PFKP, PDHA1 or SDHA, increasing glycolysis and oxidative phosphorylation. Which, with the let-7 repression may enhance tissue repair in adult tissue (PubMed:24209617).