SDS-Page
(Detection of AG-2: Cell and tissue lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 1ug/ml sheep anti-hAG-2.)
Sheep anti-Human Anterior Gradient 2 Polyclonal Antibody | anti-AGR2 antibody
Anterior Gradient 2 (Anterior Gradient Protein 2 Homolog, AG-2, hAG-2, HPC8, Secreted Cement Gland Protein XAG-2 Homolog, AGR2, AG2, UNQ515/PRO1030)
Gene Names
AGR2; AG2; GOB-4; HAG-2; XAG-2; PDIA17
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
Anterior Gradient 2; Polyclonal Antibody; Anterior Gradient 2 (Anterior Gradient Protein 2 Homolog; AG-2; hAG-2; HPC8; Secreted Cement Gland Protein XAG-2 Homolog; AGR2; AG2; UNQ515/PRO1030); Anti -Anterior Gradient 2 (Anterior Gradient Protein 2 Homolog; anti-AGR2 antibody
Host
Sheep
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human AG-2.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with sterile PBS.
Applicable Applications for anti-AGR2 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Dilution: Western Blot: 1ug/ml
Immunohistochemistry: 5-15ug/ml
ELISA: 0.5-1ug/ml
Dilution: Western Blot: 1ug/ml
Immunohistochemistry: 5-15ug/ml
ELISA: 0.5-1ug/ml
Immunogen
Purified, E. coli-derived, recombinant human AG-2 (aa21-175; Accession # O95994).
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS, 0.02% sodium azide. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SDS-Page
(Detection of AG-2: Cell and tissue lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 1ug/ml sheep anti-hAG-2.)
SDS-Page
(Detection of AG-2: Cell and tissue lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 1ug/ml sheep anti-hAG-2.)
Related Product Information for anti-AGR2 antibody
AG-2 (Anterior gradient protein 2; also AGR2, GOB4 and HPC8) is an 18-21kD member of the PDI family of enzymes. It is expressed in secretory cell types, such as prostate epithelium, small intestine goblet, Paneth and enteroendocrine cells, and multiple carcinoma cell types. Although AG-2 possesses a signal sequence, it appears to be rarely secreted, instead being found in the ER due the presence of a KTEL ER retention signal at its C-terminus. AG-2 forms transient disulfide linkages with molecules destined for secretion, possibly aiding protein folding. Mature human AG-2 is 155aa in length (aa21-175). Cys81 is presumed to participate in intermolecular bond formation. There is one potential alternative start site 20aa upstream of the standard start site.
Product Categories/Family for anti-AGR2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
19,979 Da
NCBI Official Full Name
anterior gradient protein 2 homolog
NCBI Official Synonym Full Names
anterior gradient homolog 2 (Xenopus laevis)
NCBI Official Symbol
AGR2
NCBI Official Synonym Symbols
AG2; GOB-4; HAG-2; XAG-2; PDIA17
NCBI Protein Information
anterior gradient protein 2 homolog; AG-2; HPC8; OTTHUMP00000116014; OTTHUMP00000201755; anterior gradient 2 homolog; secreted cement gland homolog; secreted cement gland protein XAG-2 homolog; protein disulfide isomerase family A, member 17
UniProt Protein Name
Anterior gradient protein 2 homolog
Protein Family
UniProt Gene Name
AGR2
UniProt Synonym Gene Names
AG2
UniProt Entry Name
AGR2_HUMAN
Uniprot Description
AGR2: Required for MUC2 post-transcriptional synthesis and secretion. May play a role in the production of mucus by intestinal cells. Proto-oncogene that may play a role in cell migration, cell differentiation and cell growth. Interacts with LYPD3 and DAG1 (alphaDAG1). Interacts with MUC2; disulfide-linked. Expressed strongly in trachea, lung, stomach, colon, prostate and small intestine. Expressed weakly in pituitary gland, salivary gland, mammary gland, bladder, appendix, ovary, fetal lung, uterus, pancreas, kidney, fetal kidney, testis, placenta, thyroid gland and in estrogen receptor (ER)-positive breast cancer cell lines. Belongs to the AGR family.
Protein type: Oncoprotein; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 7p21.3
Cellular Component: mitochondrion; endoplasmic reticulum; extracellular region
Molecular Function: protein binding
Research Articles on AGR2
Similar Products
Product Notes
The AGR2 agr2 (Catalog #AAA627087) is an Antibody produced from Sheep and is intended for research purposes only. The product is available for immediate purchase. The Anterior Gradient 2 (Anterior Gradient Protein 2 Homolog, AG-2, hAG-2, HPC8, Secreted Cement Gland Protein XAG-2 Homolog, AGR2, AG2, UNQ515/PRO1030) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Anterior Gradient 2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Dilution: Western Blot: 1ug/ml Immunohistochemistry: 5-15ug/ml ELISA: 0.5-1ug/ml. Researchers should empirically determine the suitability of the AGR2 agr2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Anterior Gradient 2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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