Rabbit anti-Pig Angiopoietin Like Protein 4 (ANGPTL4) Polyclonal Antibody | anti-ANGPTL4 antibody

Polyclonal Antibody to Angiopoietin Like Protein 4 (ANGPTL4)

Cat. Num. AAA2002726

• Gene Names: ANGPTL4; PGAR
• Reactivity: Pig
• Applications: Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
• Purity: Affinity Chromatography
• Synonyms: Angiopoietin Like Protein 4 (ANGPTL4); Polyclonal Antibody; Polyclonal Antibody to Angiopoietin Like Protein 4 (ANGPTL4); anti-ANGPTL4 antibody

• Host: Rabbit
• Reactivity: Pig
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against ANGPTL4. It has been selected for its ability to recognize ANGPTL4 in immunohistochemical staining andwestern blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
• Concentration: 500ug/mL (varies by lot)
• Sequence: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.; MGHHHHHHSGS- DGGW TVIQRRQDGS VDFNQPWEAY KDGFGDPKGE FWLGLEKVHR IMGDRGSRLA VQLQDWEGNA ESLQFPVHLG GEDTAYSLQL TAPVASKLGA TIDTPSGLSL PFSTWDQDHD LRGDKNCAKI LSGGWWFGTC SHSNLNGQYF HSIPRQREQR KKGIFWKTW
• Sequence Length: 368

• Applicable Applications for anti-ANGPTL4 antibody: Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Western blotting: 1:100-400; Immunocytochemistry in formalin fixed cells: 1:100-500; Immunohistochemistry in formalin fixed frozen section: 1:100-500; Immunohistochemistry in paraffin section: 1:50-200; Enzyme-linked Immunosorbent Assay: 1:100-200
• Immunogen: Recombinant ANGPTL4 (Asp227~Trp389) expressed in E.coli.
• Cross Reactivity: Pig
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2050603
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant ANGPTL4, Porcine.)

NCBI and Uniprot Product Information

• NCBI GI #: 84579913
• NCBI GeneID: 397628
• NCBI Accession #: NP_001033733.1
• NCBI GenBank Nucleotide #: NM_001038644.1
• UniProt Accession #: Q2TNK5; Q1H5H6; Q7YS01
• Molecular Weight: 45,534 Da
• NCBI Official Full Name: angiopoietin-related protein 4
• NCBI Official Symbol: ANGPTL4
• NCBI Official Synonym Symbols: PGAR
• NCBI Protein Information: angiopoietin-related protein 4
• UniProt Protein Name: Angiopoietin-related protein 4
• Protein Family: Angiopoietin-related protein
• UniProt Gene Name: ANGPTL4
• UniProt Synonym Gene Names: PGAR

Uniprot Description

Protein with hypoxia-induced expression in endothelial cells. May act as a regulator of angiogenesis and modulate tumorigenesis. Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. May exert a protective function on endothelial cells through an endocrine action. It is directly involved in regulating glucose homeostasis, lipid metabolism, and insulin sensitivity. In response to hypoxia, the unprocessed form of the protein accumulates in the subendothelial extracellular matrix (ECM). The matrix-associated and immobilized unprocessed form limits the formation of actin stress fibers and focal contacts in the adhering endothelial cells and inhibits their adhesion. It also decreases motility of endothelial cells and inhibits the sprouting and tube formation ().