Typical Testing Data/Standard Curve (for reference only)
Rat Alpha ketoglutae-dependent dioxygenase FTO (FTO) ELISA Kit | FTO elisa kit
Rat Alpha ketoglutae-dependent dioxygenase FTO (FTO) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: FTO ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-FTO antibody and an FTO-HRP conjugate. The assay sample and buffer are incubated together with FTO-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FTO concentration since FTO from samples and FTO-HRP conjugate compete for the anti-FTO antibody binding site. Since the number of sites is limited, as more sites are occupied by FTO from the sample, fewer sites are left to bind FTO-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FTO concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene is a nuclear protein of the AlkB related non-haem iron and 2-oxoglutarate-dependent oxygenase superfamily but the exact physiological function of this gene is not known. Other non-heme iron enzymes function to reverse alkylated DNA and RNA damage by oxidative demethylation. Studies in mice and humans indicate a role in nervous and cardiovascular systems and a strong association with body mass index, obesity risk, and type 2 diabetes. [provided by RefSeq, Jul 2011]
Uniprot Description
FTO: Dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation. Has highest activity towards single- stranded RNA containing 3-methyluracil, followed by single- stranded DNA containing 3-methylthymine. Has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine. Has no activity towards 1-methylguanine. Has no detectable activity towards double-stranded DNA. Requires molecular oxygen, alpha-ketoglutarate and iron. Contributes to the regulation of the global metabolic rate, energy expenditure and energy homeostasis. Contributes to the regulation of body size and body fat accumulation. Defects in FTO are the cause of growth retardation developmental delay coarse facies and early death (GDFD). A severe polymalformation syndrome characterized by postnatal growth retardation, microcephaly, severe psychomotor delay, functional brain deficits and characteristic facial dysmorphism. In some patients, structural brain malformations, cardiac defects, genital anomalies, and cleft palate are observed. Early death occurs by the age of 3 years. Belongs to the fto family. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 1.14.11.-
Chromosomal Location of Human Ortholog: 16q12.2
Cellular Component: nuclear speck; nucleus
Molecular Function: ferrous iron binding; DNA-N1-methyladenine dioxygenase activity
Biological Process: DNA dealkylation; RNA repair; regulation of multicellular organism growth; thermoregulation
Disease: Growth Retardation, Developmental Delay, Coarse Facies, And Early Death
Research Articles on FTO
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Product Notes
The Rat FTO fto (Catalog #AAA7228899) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7228899 ELISA Kit recognizes Rat FTO. It is sometimes possible for the material contained within the vial of "Alpha ketoglutae-dependent dioxygenase FTO (FTO), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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